An 8-week trial investigated how andrographolide effected lipopolysaccharide (LPS)-induced serum biochemical indices, immune responses and the intestinal inflammatory response of Monopterus albus. The study included control and andrographolide groups (150 mg/kg andrographolide) with three replicates per group. Samples were taken 0, 3, 6, 12, 24 and 48 h after LPS injection. The results showed that the levels of GLU, COR, AST, ALT, IgM, LZM, AKP, C3 and C4 in serum showed an initial increasing that then decreased. Compared with that of the control group, the levels of serum LZM, AKP, IgM, C3 and C4 in the andrographolide group increased quicker after LPS injection, while the levels of GLU, COR, AST and ALT increased more slowly; further, these indices recovered to normal levels quicker. The expression of several genes in the intestine first increased and then decreased after LPS injection. Compared with that of the control group, il-10, tgf-β1, tgf-β2 and tgf-β3 gene expression in the andrographolide group increased quicker after LPS injection, while that of il-1β, il-8, il-12β, nf-κb, tlr-3, tlr-7 and tlr-8 increased more slowly; these indices recovered to normal levels more quickly. In conclusion, LPS injection induced immune stress and inflammatory response in M. albus. Dietary supplementation with 150 mg/kg andrographolide effectively alleviated these responses.

中文翻译：

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穿心莲内酯对脂多糖诱导血清生化指标、免疫反应及肠道炎症的影响与黄鹂基因表达相关

一项为期 8 周的试验研究了穿心莲内酯如何影响脂多糖 (LPS) 诱导的血清生化指标、免疫反应和白头翁的肠道炎症反应. 该研究包括对照组和穿心莲内酯组（150 mg/kg 穿心莲内酯），每组重复 3 次。在 LPS 注射后 0、3、6、12、24 和 48 小时取样。结果表明，血清中GLU、COR、AST、ALT、IgM、LZM、AKP、C3和C4的含量呈先升高后降低的趋势。与对照组相比，注射LPS后穿心莲内酯组血清LZM、AKP、IgM、C3、C4水平升高较快，而GLU、COR、AST、ALT水平升高较慢；此外，这些指数更快地恢复到正常水平。注射LPS后，肠道中几种基因的表达先升高后降低。与对照组相比, il-10 , tgf-β1 , tgf-β2和TGF-β3在穿心莲内酯组基因表达LPS注射后增加更快，而的IL-1β，IL-8 ，IL-12β，NF - κB，TLR-3 ，TLR-7和TLR-8增加更慢; 这些指数更快地恢复到正常水平。总之，LPS 注射诱导了M 的免疫应激和炎症反应。 阿不思。膳食补充剂 150 mg/kg 穿心莲内酯可有效缓解这些反应。