ABSTRACT

The study assessed the individual and interactive role of acetylsalicylic acid (ASA) and nitric oxide (NO) against excess copper (Cu) concentrations. Excess Cu significantly reduced growth and photosynthesis, although the application of ASA and NO significantly mitigated the reduction with much visible effect in their combination. The oxidative effects of Cu have been evident as increased reactive oxygen species (ROS) production, resulting in increased malondialdehyde (MDA) and reduced membrane stability index (MSI). Both ASA and NO reduced oxidative damage obvious as reduced MDA and lipoxygenase (LOX) down-regulation. Up-regulation of enzymatic and non-enzymatic antioxidants due to ASA and NO application contributed to the quick elimination of excess ROS. Oxidative effects of excess Cu were mitigated by osmolyte accumulation, up-regulation of the γ-Glutamyl kinase (γ -GK), glyoxalase, and down-regulation of proline oxidase (PROX). Reduced Cu accumulation in our study showed the crosstalk effect of ASA and NO resulting in the protection of mung bean growth against the damaging effects of Cu.

摘要

该研究评估了乙酰水杨酸（ASA）和一氧化氮（NO）对过量铜（Cu）浓度的个体和相互作用作用。过量的铜显着降低了生长和光合作用，尽管ASA和NO的应用显着减轻了还原作用，并在它们的组合中产生了明显的效果。Cu的氧化作用已被证明是增加了活性氧（ROS）的产生，从而导致了丙二醛（MDA）的增加和膜稳定性指数（MSI）的降低。ASA和NO均减少了氧化损伤，因为MDA和脂氧合酶（LOX）的下调降低了。由于ASA和NO的应用，酶抗氧化剂和非酶抗氧化剂的上调有助于快速消除过量的ROS。渗透液的积累减轻了过量铜的氧化作用，γ-谷氨酰激酶（γ-GK），乙二醛酶的上调和脯氨酸氧化酶（PROX）的下调。在我们的研究中，减少的铜积累表明ASA和NO的串扰效应可保护绿豆免受铜的破坏。