1-Deoxynojirimycin (DNJ) is a potent inhibitor of α-glucosidase having antidiabetic and antiviral activities. In the present study, DNJ production by Bacillus subtilis subsp. inaquosorum KCTC 13429 (B. subtilis IWT) was confirmed and a mutant B. subtilis I.247 strain showing 50% increased DNJ production than wild-type strain after cultivation in 5% defatted soybean meal (DFS) for five days was isolated by UV random mutagenesis. The optimum culture conditions to maximize DNJ production by B. subtilis I.247 was predicted using response surface methodology to cultivate in medium containing 3.4% sorbitol and 2.4% yeast extract as carbon and nitrogen sources, respectively, at a temperature of 32°C. Under these conditions B. subtilis I.247 was able to produce 359 mg/L after five days of cultivation. Furthermore, when the B. subtilis I.247 transformant harboring a vector expressing a gabT1-yktc1-gutB1 DNJ biosynthetic gene cluster was cultured under the optimized condition, DNJ production was increased to 773 mg/L, representing a level 6.2-fold higher than that of the wild-type strain cultured in 5% DFS for five days.

1-脱氧野rim霉素（DNJ）是一种具有抗糖尿病和抗病毒活性的强力α-葡萄糖苷酶抑制剂。在本研究中，枯草芽孢杆菌亚种产生的DNJ 。确认了inaquosorum KCTC 13429（枯草芽孢杆菌IWT），并在5％脱脂豆粕（DFS）中培养5天后，显示出比野生型菌株DNJ产量增加50％的突变枯草芽孢杆菌I.247菌株随机诱变。使枯草芽孢杆菌产生DNJ最大化的最佳培养条件使用响应表面方法预测I.247在32°C的温度下分别包含3.4％山梨糖醇和2.4％酵母提取物作为碳和氮源的培养基中进行培养。在这些条件下，枯草芽孢杆菌I.247在培养五天后能够产生359 mg / L。此外，在优化条件下培养带有表达gabT1-yktc1-gutB1 DNJ生物合成基因簇的载体的枯草芽孢杆菌I.247转化体时，DNJ产量增加到773 mg / L，比水平高6.2倍。在5％DFS中培养5天的野生型菌株的抗性。