Mouse embryonic stem cells (ESCs) are derived from the inner cell mass of blastocyst-stage embryos and cultured in different culture media with varied pluripotency. Sporadically, a small population of ESCs exhibit 2-cell stage embryonic features in serum containing medium. However, whether ESCs can transit into 2-cell embryo-like (2C-like) cells in the chemically defined media remains largely unknown.

中文翻译：

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视黄酸诱导 NELFA 介导的小鼠胚胎干细胞的 2C 样状态与化学成分确定培养基中的表观遗传修饰和代谢过程相关

小鼠胚胎干细胞 (ESC) 来源于囊胚期胚胎的内细胞团，并在具有不同多能性的不同培养基中培养。偶尔，一小群 ESC 在含血清的培养基中表现出 2 细胞阶段的胚胎特征。然而，胚胎干细胞是否可以在化学成分确定的培养基中转变为 2 细胞胚胎样（2C 样）细胞仍然很大程度上未知。