The aim of this work was to develop a simple, green and low-consumption method for the extraction of ganoderic acids. An in situ effervescence reaction-assisted mechanochemical extraction method was proposed, and the effects of independent variations in terms of sodium bicarbonate concentration, milling time, and liquid/solid ratio (mL/g) were investigated by means of a response surface methodology (RSM) Box-Behnken design (BBD). The highest yields of ganoderic acids were achieved by grinding a Ganoderma lucidum sample with sodium bicarbonate (372.60 mg/g) and citric acid for 0.87 min with a water to material ratio of 10.58 mL. The linear calibration ranges were from 0.01–5 μg/mL for the six analytes and had good linearity with a correlation coefficient of determination higher than 0.9991. In addition, the obtained LODs of the six target analytes were 1.46 − 2.97 ng/mL, and the LOQs calculated as ten times the signal-to-noise ratio were 4.88 − 9.90 ng/mL. The proposed method was successfully applied to the Ganoderma lucidum samples.

这项工作的目的是开发一种简单，绿色和低消耗量的灵芝酸提取方法。提出了一种起泡反应辅助的机械化学萃取方法，并通过响应面法（RSM）研究了碳酸氢钠浓度，研磨时间和液/固比（mL / g）的独立变化的影响。 ）Box-Behnken设计（BBD）。通过研磨灵芝可获得最高的灵芝酸产量样品用碳酸氢钠（372.60 mg / g）和柠檬酸处理0.87分钟，水/材料比为10.58 mL。六种分析物的线性校准范围为0.01-5μg/ mL，并具有良好的线性，测定的相关系数高于0.9991。此外，获得的六种目标分析物的LOD为1.46-2.97 ng / mL，计算出的信噪比的十倍的LOQ为4.88-9.90 ng / mL。所提出的方法已成功应用于灵芝样品。