Purpose

Over the last five decades, there has been rapid growth in the production of poultry livestock, processing, marketing and consumption globally. This work investigates on the usage of biotechnological tools such as fermentation and enzymatic hydrolysis to prepare hydrolysates from the poultry processing industry by-product, i.e. chicken liver.

Methods

Fermentatively (FCLH) and enzymatically (ECLH) prepared chicken liver hydrolysates (CLHs) were evaluated for their ability to alleviate ill effects of anaemia induced in mice models.

Results

The iron content (mg/100 g) of FCLH and ECLH was 16.21 and 17.35, respectively. The blood haemoglobin content (15.47 ± 0.43 g/dL) of animals reduced by 50.79 ± 2.78%, upon induction of anaemia apart from ~ 11% weight loss (p < 0.05) in different test groups. Oral administration of FCLH and ECLH restored the haemoglobin content of animals in the respective groups from > 80 to 95% of the levels before induction of anaemia (p < 0.05), as compared to haemoglobin deficient conditions in iron free diet fed group. Oral feeding of CLHs, apart from restoring the haemoglobin content and the lost weight, also improved the antioxidative status of the serum significantly (p < 0.05).

Conclusion

Overall, this study clearly demonstrates the ability of CLHs (FCLH or ECLH) to alleviate the effects of cyclophosphamide induced anaemia. This is the first report of alleviation of iron deficiency anaemia by CLHs and suggests the potential of CLHs as a nutraceutical.

Graphic Abstract

中文翻译：

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使用生物技术工具从鸡肝中制备生物活性化合物的体内抗厌食作用

目的

在过去的五十年中，全球范围内禽类牲畜的生产，加工，销售和消费量一直在快速增长。这项工作研究了利用生物技术工具（例如发酵和酶促水解）从家禽加工业副产品即鸡肝中制备水解产物的情况。

方法

评估了发酵（FCLH）和酶法（ECLH）制备的鸡肝水解产物（CLHs）减轻小鼠模型中引起的贫血不良影响的能力。

结果

FCLH和ECLH的铁含量（mg / 100 g）分别为16.21和17.35。在不同的试验组中，除引起约11％的体重减轻（p <0.05）外，诱发贫血的动物的血液血红蛋白含量（15.47±0.43 g / dL）降低了50.79±2.78％。与无铁饮食喂养组的血红蛋白缺乏症相比，口服FCLH和ECLH可使各组动物的血红蛋白含量从诱导贫血前的水平的> 80％降至95％（p <0.05）。口服补充CLHs不仅可以恢复血红蛋白含量和减轻体重，还可以显着改善血清的抗氧化状态（p <0.05）。

结论

总体而言，这项研究清楚地证明了CLH（FCLH或ECLH）减轻环磷酰胺引起的贫血的作用。这是首次通过CLH缓解铁缺乏性贫血的报道，表明CLH作为营养保健品的潜力。

图形摘要