An 8-week feeding trial was designed to evaluate the potential of cricket meal, Gryllus bimaculatus, as a locally available unconventional source of protein in a practical diet for olive flounder, Paralichthys olivaceus. Three hundred juvenile fish (Initial weight, 33.5 ± 0.01 g) were randomly distributed into five dietary groups in triplicates (20 fish per tank) and each group was hand-fed one of the experimental diets containing graded level of a cricket meal (CR) replacing 0, 20, 40, 60, and 80% of fish meal (FM) protein (designated as CR0, CR20, CR40, CR60, and CR80). Although replacing more than 40% of dietary FM protein resulted in lower growth rates and feed utilization efficiency, juvenile flounders fed the highest level of dietary CR (CR80) still performed as well as control group (CR0). Replacing more than 20% of FM with CR markedly reduced apparent nutrient digestibility in fish. Plasma total cholesterol concentration showed decreasing trend with increasing inclusion levels of CR in the diet. Whole body and fillet lipid content decreased with increasing levels of dietary CR. There was a remarkable enhancement in the levels of C18 fatty acids and reduction in the levels of n − 3 highly unsaturated fatty acids in the fillets of fish fed with the CR diets, which became more prominent with greater levels of dietary FM replacement. Antioxidant enzyme activities tended to increase with increasing dietary CR levels but gradually decreased when the replacement level exceeded 60%. Overall, the efficiency of CR as a promising substitute for FM in the flounder diets has been confirmed not only in relation to growth rates, but also in terms of immunopotency.

中文翻译：

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蟋蟀、Gryllus bimaculatus、鱼粉替代橄榄比目鱼、Paralichthys olivaceus、幼鱼的营养评价

一项为期 8 周的喂养试验旨在评估蟋蟀粉Gryllus bimaculatus作为当地可用的非常规蛋白质来源，用于橄榄比目鱼Paralichthys olivaceus的实用饮食中的潜力. 三百条幼鱼（初始体重，33.5 ± 0.01 克）随机分成五个饮食组，一式三份（每罐 20 条鱼），每组手工喂养一种含有分级水平的蟋蟀粉 (CR) 的实验饮食替代 0、20、40、60 和 80% 的鱼粉 (FM) 蛋白质（指定为 CR0、CR20、CR40、CR60 和 CR80）。尽管替代超过 40% 的日粮 FM 蛋白导致较低的生长率和饲料利用效率，但饲喂最高水平日粮 CR (CR80) 的幼鱼仍与对照组 (CR0) 一样好。用 CR 代替 20% 以上的 FM 显着降低了鱼的表观营养消化率。随着饮食中 CR 含量的增加，血浆总胆固醇浓度呈下降趋势。全身和鱼片脂质含量随着日粮 CR 水平的增加而降低。C18 脂肪酸水平显着提高，而n  - 3 高度不饱和脂肪酸在用 CR 日粮喂养的鱼的鱼片中，随着日粮 FM 替代水平的提高而变得更加突出。抗氧化酶活性随着日粮CR水平的增加而增加，但当替代水平超过60%时逐渐降低。总体而言，CR 作为比目鱼日粮中 FM 的有希望替代品的效率不仅在生长速度方面得到证实，而且在免疫效力方面也得到了证实。