A modular approach was applied for the synthesis of bienzyme-polymer nanoconjugates (nano-BECs) (50–70 nm) consisting of two enzymes (carbonyl reductase and glucose dehydrogenase) conjugated within a single universal polymer scaffold. The amount of the product ethyl (R)-2-hydroxy-4-phenylbutyrate (R-HPBE) with nano-BECs as the catalyst was 533 mM in a dibutyl phthalate-phosphate buffer (dibutyl phthalate-PB) biphasic system, while the amount of R-HPBE was 349 mM using carbonyl reductase-poly(acrylic acid) as the catalyst, indicating that the nano-BECs have an advantage for coenzyme regeneration. Compared with a single aqueous phase, the substrate treatment capacity was improved at the interface of the dibutyl phthalate-PB biphasic system. Under the optimal reaction conditions (35 °C, 40 h, dibutyl phthalate-PB 1: 1), nano-BECs can completely convert substrate into optically pure R-HPBE (enantiomeric excess (e.e.) >99.9%) in the organic-aqueous system.

一种模块化方法被应用于合成双酶聚合物纳米共轭物（nano-BEC）（50-70 nm），该共轭物由两种酶（羰基还原酶和葡萄糖脱氢酶）共轭在一个通用聚合物支架中组成。在邻苯二甲酸二丁酯-磷酸盐缓冲液（邻苯二甲酸二丁酯-PB）双相体系中，以纳米BEC为催化剂的（R）-2-羟基-4-苯基丁酸乙酯（R-HPBE）的量为533 mM。以羰基还原酶-聚（丙烯酸）为催化剂，R-HPBE的量为349 mM，表明纳米BEC具有辅酶再生的优势。与单一水相相比，邻苯二甲酸二丁酯-PB双相体系界面处的底物处理能力有所提高。在最佳反应条件下（35°C，40 h，邻苯二甲酸二丁酯-PB 1：1），