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Regenerated coenzyme-based preparation of bienzyme-polymer nanoconjugates and their applications for the synthesis of ethyl (R)-2-hydroxy-4-phenylbutyrate
Korean Journal of Chemical Engineering ( IF 2.9 ) Pub Date : 2021-05-06 , DOI: 10.1007/s11814-021-0775-7
Yuan Lu , Hongqian Dai , Pengpeng Cheng , Hanbing Shi , Lan Tang , Xingyuan Sun , Zhimin Ou

A modular approach was applied for the synthesis of bienzyme-polymer nanoconjugates (nano-BECs) (50–70 nm) consisting of two enzymes (carbonyl reductase and glucose dehydrogenase) conjugated within a single universal polymer scaffold. The amount of the product ethyl (R)-2-hydroxy-4-phenylbutyrate (R-HPBE) with nano-BECs as the catalyst was 533 mM in a dibutyl phthalate-phosphate buffer (dibutyl phthalate-PB) biphasic system, while the amount of R-HPBE was 349 mM using carbonyl reductase-poly(acrylic acid) as the catalyst, indicating that the nano-BECs have an advantage for coenzyme regeneration. Compared with a single aqueous phase, the substrate treatment capacity was improved at the interface of the dibutyl phthalate-PB biphasic system. Under the optimal reaction conditions (35 °C, 40 h, dibutyl phthalate-PB 1: 1), nano-BECs can completely convert substrate into optically pure R-HPBE (enantiomeric excess (e.e.) >99.9%) in the organic-aqueous system.



中文翻译:

基于再生辅酶的双酶-聚合物纳米共轭物的制备及其在(R)-2-羟基-4-苯基丁酸乙酯合成中的应用

一种模块化方法被应用于合成双酶聚合物纳米共轭物(nano-BEC)(50-70 nm),该共轭物由两种酶(羰基还原酶和葡萄糖脱氢酶)共轭在一个通用聚合物支架中组成。在邻苯二甲酸二丁酯-磷酸盐缓冲液(邻苯二甲酸二丁酯-PB)双相体系中,以纳米BEC为催化剂的(R)-2-羟基-4-苯基丁酸乙酯(R-HPBE)的量为533 mM。以羰基还原酶-聚(丙烯酸)为催化剂,R-HPBE的量为349 mM,表明纳米BEC具有辅酶再生的优势。与单一水相相比,邻苯二甲酸二丁酯-PB双相体系界面处的底物处理能力有所提高。在最佳反应条件下(35°C,40 h,邻苯二甲酸二丁酯-PB 1:1),

更新日期:2021-05-07
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