Gene-based mapping of trehalose biosynthetic pathway genes reveals association with source- and sink-related yield traits in a spring wheat panel,Food and Energy Security

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Gene-based mapping of trehalose biosynthetic pathway genes reveals association with source- and sink-related yield traits in a spring wheat panel
Food and Energy Security ( IF 4.0 ) Pub Date : 2021-05-07 , DOI: 10.1002/fes3.292
Danilo H Lyra ₁ , Cara A Griffiths ₂ , Amy Watson ₂ , Ryan Joynson ₃ , Gemma Molero ₄ , Alina-Andrada Igna ₂ , Keywan Hassani-Pak ₁ , Matthew P Reynolds ₄ , Anthony Hall ₃ , Matthew J Paul ₂

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Trehalose 6-phosphate (T6P) signalling regulates carbon use and allocation and is a target to improve crop yields. However, the specific contributions of trehalose phosphate synthase (TPS) and trehalose phosphate phosphatase (TPP) genes to source- and sink-related traits remain largely unknown. We used enrichment capture sequencing on TPS and TPP genes to estimate and partition the genetic variation of yield-related traits in a spring wheat (Triticum aestivum) breeding panel specifically built to capture the diversity across the 75,000 CIMMYT wheat cultivar collection. Twelve phenotypes were correlated to variation in TPS and TPP genes including plant height and biomass (source), spikelets per spike, spike growth and grain filling traits (sink) which showed indications of both positive and negative gene selection. Individual genes explained proportions of heritability for biomass and grain-related traits. Three TPS1 homologues were particularly significant for trait variation. Epistatic interactions were found within and between the TPS and TPP gene families for both plant height and grain-related traits. Gene-based prediction improved predictive ability for grain weight when gene effects were combined with the whole-genome markers. Our study has generated a wealth of information on natural variation of TPS and TPP genes related to yield potential which confirms the role for T6P in resource allocation and in affecting traits such as grain number and size confirming other studies which now opens up the possibility of harnessing natural genetic variation more widely to better understand the contribution of native genes to yield traits for incorporation into breeding programmes.

中文翻译：

海藻糖生物合成途径基因的基因定位揭示了春小麦面板中与源和库相关的产量性状的关联

海藻糖 6-磷酸 (T6P) 信号调节碳的使用和分配，是提高作物产量的目标。然而，海藻糖磷酸合酶 (TPS) 和海藻糖磷酸磷酸酶 (TPP) 基因对源和汇相关性状的具体贡献仍然很大程度上未知。我们使用 TPS 和 TPP 基因的富集捕获测序来估计和划分春小麦（Triticum aestivum) 专门建立的育种面板，用于捕获 75,000 个 CIMMYT 小麦品种集合中的多样性。12 种表型与 TPS 和 TPP 基因的变异相关，包括植物高度和生物量（来源）、每个穗的小穗数、穗生长和籽粒灌浆性状（汇），它们显示出阳性和阴性基因选择的迹象。单个基因解释了生物量和谷物相关性状的遗传比例。三台TPS1同源物对性状变异特别重要。在植物高度和谷物相关性状的 TPS 和 TPP 基因家族内部和之间发现了上位相互作用。当基因效应与全基因组标记相结合时，基于基因的预测提高了对粒重的预测能力。我们的研究产生了大量关于与产量潜力相关的 TPS 和 TPP 基因自然变异的信息，这证实了 T6P 在资源分配和影响谷物数量和大小等性状方面的作用，这证实了其他研究现在开辟了利用的可能性更广泛地了解自然遗传变异，以更好地了解天然基因对产生可纳入育种计划的性状的贡献。

更新日期：2021-05-07

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