Establishment of a novel double-monoclonal antibody sandwich enzyme-linked immunosorbent assay (ELISA): tool for human B7-H4 detection in autoimmune diseases,Clinical & Experimental Immunology

当前位置： X-MOL 学术 › Clin. Exp. Immunol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Establishment of a novel double-monoclonal antibody sandwich enzyme-linked immunosorbent assay (ELISA): tool for human B7-H4 detection in autoimmune diseases
Clinical & Experimental Immunology ( IF 3.4 ) Pub Date : 2021-05-07 , DOI: 10.1111/cei.13610
Sisi Ding _{1,

2} , Hengxin Zhou _{1,

2} , Yanzheng Gu _{1,

2} , Yu Shen _{1,

2} , Li Zhang ₃ , Huayang Zhao ₃ , Jian Wu ₁ , Xueguang Zhang ₁ , Xin Chang ₁ , Cuiping Liu _{1,

2}

Affiliation

B7-H4, one of the immunoregulatory proteins, plays an inhibitory role by inhibiting T cell proliferation and cytokine production. Nevertheless, the significance of soluble B7-H4 (sB7-H4) in autoimmune diseases is unclear. In our study, we developed two novel mouse anti-human B7-H4 monoclonal antibodies (mAbs) (clones 8D4 and 7E1) with utilities for flow cytometry, immunoblotting and immunofluorescence. We characterized 7E1 as a functional antibody with antagonistic activity, which could promote T cell proliferation and regulate cytokine production. Furthermore, based on the different epitope specificities, we established a novel enzyme-linked immunosorbent assay (ELISA) which could detect sB7-H4 sensitively and specifically. Using this ELISA kit, sB7-H4 was observed in a high proportion of autoimmune diseases patients. We found that the levels of sB7-H4 were significantly higher in patients with systemic lupus erythematosus (SLE), type I diabetes (T1D) and Graves’ disease (GD). Together, sB7-H4 in human serum is regarded not only as a regulator of T cell activation but may also be a diagnostic marker of autoimmune diseases.

中文翻译：

一种新型双单克隆抗体夹心酶联免疫吸附试验（ELISA）的建立：自身免疫性疾病中人B7-H4检测的工具

B7-H4是一种免疫调节蛋白，通过抑制T细胞增殖和细胞因子产生而发挥抑制作用。然而，可溶性 B7-H4 (sB7-H4) 在自身免疫性疾病中的意义尚不清楚。在我们的研究中，我们开发了两种新型小鼠抗人 B7-H4 单克隆抗体 (mAb)（克隆 8D4 和 7E1），可用于流式细胞术、免疫印迹和免疫荧光。我们将 7E1 定性为具有拮抗活性的功能性抗体，可促进 T 细胞增殖并调节细胞因子的产生。此外，基于不同的表位特异性，我们建立了一种新的酶联免疫吸附试验（ELISA），可以灵敏、特异地检测sB7-H4。使用该 ELISA 试剂盒，在高比例的自身免疫性疾病患者中观察到 sB7-H4。我们发现系统性红斑狼疮 (SLE)、I 型糖尿病 (T1D) 和格雷夫斯病 (GD) 患者的 sB7-H4 水平显着升高。总之，人血清中的 sB7-H4 不仅被认为是 T 细胞活化的调节剂，而且可能是自身免疫性疾病的诊断标志物。

更新日期：2021-07-12

点击分享查看原文

点击收藏

阅读更多本刊最新论文