Biocatalysts provide many advantages over the traditional chemically assisted processes prevalent in industries. Consequently, the search for novel enzymes has increased over the years with a renewed interest in thermophilic and psychrophilic bacterial species. Enzymes or extremozymes extracted from such species have exhibited an affinity to extreme temperatures which is a prerequisite for many industrial applications. However, utilisation of these enzymes faces a major bottleneck. The distribution of sequence data associated with thermophiles and psychrophiles is overwhelming, spanning various databases and scientific literature. Based on more than 100 publications and genomes from over 300 thermophilic and psychrophilic bacterial species, we have constructed the database IND-Enzymes (indenzymes.srmist.edu.in). This database consists of over 20,120 nucleotide and protein sequences belonging to the hydrolytic enzyme class lipase, protease, esterase and amylase. Users can access over 100 published enzymes, 200 PDB structural data. Enzymes derived from genomes can be directly downloaded and users can also access the entire annotation data derived from species individually. Along with an alignment tool and python based pipelines, IND-Enzymes serves as the largest sequence repository for hydrolytic enzymes from thermophilic and psychrophilic bacterial species. This database showcases resources that are essential for protein engineering of hot–cold stable enzymes.

与工业上普遍使用的传统化学辅助工艺相比，生物催化剂具有许多优势。因此，随着对嗜热和嗜热细菌种类的新的兴趣，近年来对新型酶的搜索增加了。从此类物种中提取的酶或极端酶表现出对极端温度的亲和力，这是许多工业应用的前提条件。但是，这些酶的利用面临主要瓶颈。与嗜热菌和嗜冷菌有关的序列数据的分布是压倒性的，涵盖了各种数据库和科学文献。基于300多个嗜热和嗜热细菌物种的100多个出版物和基因组，我们构建了IND-酶数据库（indenzymes.srmist.edu.in）。该数据库由属于水解酶类脂肪酶，蛋白酶，酯酶和淀粉酶的20,120多个核苷酸和蛋白质序列组成。用户可以访问100多种已发布的酶和200种PDB结构数据。可以直接下载源自基因组的酶，用户也可以单独访问源自物种的整个注释数据。与比对工具和基于python的管道一起，IND-酶可作为嗜热和嗜热细菌物种水解酶的最大序列库。该数据库展示了对热冷稳定酶的蛋白质工程必不可少的资源。