ABSTRACT

Our study aimed to determine the effect on barley (Hordeum vulgare L.) of adding potassium polyacrylate to a sandy soil under normal watering condition and drought. We focused on morphological and physiological characteristics of barley and its rhizosphere bacterial community structure using Illumina Miseq sequencing. It was found that under normal watering condition (70% of soil water holding capacity (WHC)), the soil water retention increased with the SAP addition to the soil. Barley plant height and weight, leaf relative water content and protein content decreased under drought (35% of soil WHC), the addition of SAP eliminated the negative effect of drought on plants, the proline content was reduced from 2.71 μg g⁻¹ leave dry weight with stressed plants to 0.99 μg g⁻¹ with only 0.2% of SAP. The barley rhizophere bacterial community was affected by drought. Changes in relative abundance were noticed at both phyla and OTU level. The SAP addition (0.8%) shifted the barley rhizophere bacterial community to become in part more similar to that of the normal watering condition. Also, we found that the SAP addition had a side effect itself on the barley rhizosphere bacterial community other than its capacity of water holding.

摘要

我们的研究旨在确定在正常浇水条件和干旱条件下向沙质土壤中添加聚丙烯酸钾对大麦（Hordeum vulgare L.）的影响。我们使用 Illumina Miseq 测序专注于大麦的形态和生理特征及其根际细菌群落结构。结果表明，在正常浇水条件下（土壤持水量（WHC）的 70%），土壤保水量随着 SAP 添加到土壤中而增加。大麦株高和重量、叶片相对含水量和蛋白质含量在干旱（土壤 WHC 的 35%）下降低，SAP 的加入消除了干旱对植物的负面影响，脯氨酸含量从 2.71 μg g ^{-1 叶}干燥降低受胁迫植物的重量为 0.99 μg g ^-1仅占 SAP 的 0.2%。大麦根际细菌群落受干旱影响。在门和 OTU 水平上都注意到了相对丰度的变化。SAP 添加 (0.8%) 使大麦根际细菌群落变得更类似于正常浇水条件下的细菌群落。此外，我们发现除了保水能力之外，添加 SAP 本身对大麦根际细菌群落也有副作用。