In this study, DNA was extracted from whole blood which had been collected and stored at -20°C for 5-18 years, with the aim of determining the most suitable commercial DNA extraction kit for this purpose. DNA from nine cord blood samples collected in 1999, 2001 and 2012, with low blood volumes (<1 ml), and a partly dried adult blood sample collected in 2003, having a large blood volume (6 ml) was extracted using four different DNA extraction kits: Quick-DNA Miniprep Plus kit, DNeasy Blood & Tissue kit, MagAttract HMW DNA kit and QIAamp Blood Maxi kit. We concluded that high-quality DNA can be extracted from whole blood sample collections which have been stored for even up to 18 years in a biobank at -20°C.

中文翻译：

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旧血液样本的 DNA 提取方法比较。

在这项研究中，从收集并在 -20°C 下储存 5-18 年的全血中提取 DNA，目的是确定最适合此目的的商业 DNA 提取试剂盒。从 1999、2001 和 2012 年收集的 9 个低血容量（<1 ml）脐带血样本和 2003 年收集的部分干燥的成人血样本（血容量大（6 ml））中提取的 DNA 使用四种不同的 DNA提取试剂盒：Quick-DNA Miniprep Plus 试剂盒、DNeasy Blood & Tissue 试剂盒、MagAttract HMW DNA 试剂盒和 QIAamp Blood Maxi 试剂盒。我们得出的结论是，可以从在 -20°C 的生物库中保存长达 18 年的全血样本中提取高质量的 DNA。