Umbilical cord-derived mesenchymal stem cells (UCDMSC) are attractive candidates for cell-based regenerative medicine. However, they are susceptible to replicative senescence during repetitive passaging for in-vitro expansion and induced senescence in an oxidative, inflammatory microenvironment in vivo. Aim of this study is to investigate if honey-incorporated matrices can be employed to reduce senescence of UCDMSC. Matrices were prepared by electrospinning solutions of honey with poly-vinyl alcohol (PVA). PVA:honey matrices exhibited free radical scavenging activity. Culture of UCDMSC on PVA:honey matrices showed improvement in cell proliferation compared to pure PVA nanofibres. Expression of vimentin indicated that mesenchymal phenotype is preserved after culturing on these matrices. Further, UCDMSC were serially subcultured and cells of two passages (P2 and P6) were evaluated for reactive oxygen species (ROS) load and senescence parameters. P6 cells showed a higher ROS load and β-galactosidase (β-gal) positive senescent cells compared to P2. However, culturing on PVA:honey substrates significantly reduced both ROS and β-gal markers compared to cells on PVA substrates. Honey contains several antioxidant and anti-inflammatory components, which can reduce the ROS-related senescence. Thus, it is concluded that honey containing nanofibres can be effective substrates for stem cell-based wound healing and regenerative medicine.

中文翻译：

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掺入蜂蜜的纳米纤维可减少脐带来源的间充质干细胞的复制衰老。

脐带间充质干细胞 (UCDMSC) 是基于细胞的再生医学的有吸引力的候选者。然而，它们在体外扩增的重复传代过程中容易发生复制性衰老，并在体内氧化、炎症微环境中诱导衰老。本研究的目的是调查是否可以使用掺入蜂蜜的基质来减少 UCDMSC 的衰老。通过将蜂蜜与聚乙烯醇 (PVA) 的静电纺丝溶液制备基质。PVA：蜂蜜基质表现出自由基清除活性。与纯 PVA 纳米纤维相比，在 PVA：蜂蜜基质上培养 UCDMSC 显示出细胞增殖的改善。波形蛋白的表达表明在这些基质上培养后保留了间充质表型。更远，对 UCDMSC 进行连续传代培养，并评估两代（P2 和 P6）细胞的活性氧（ROS）负荷和衰老参数。与 P2 相比，P6 细胞显示出更高的 ROS 负荷和 β-半乳糖苷酶 (β-gal) 阳性衰老细胞。然而，与 PVA 底物上的细胞相比，在 PVA：蜂蜜底物上培养显着降低了 ROS 和 β-gal 标记物。蜂蜜含有多种抗氧化和抗炎成分，可以减少与活性氧相关的衰老。因此，得出的结论是，含有纳米纤维的蜂蜜可以成为基于干细胞的伤口愈合和再生医学的有效基质。与 PVA 底物上的细胞相比，在 PVA：蜂蜜底物上培养显着降低了 ROS 和 β-gal 标记。蜂蜜含有多种抗氧化和抗炎成分，可以减少与活性氧相关的衰老。因此，得出的结论是，含有纳米纤维的蜂蜜可以成为基于干细胞的伤口愈合和再生医学的有效基质。与 PVA 底物上的细胞相比，在 PVA：蜂蜜底物上培养显着降低了 ROS 和 β-gal 标记。蜂蜜含有多种抗氧化和抗炎成分，可以减少与活性氧相关的衰老。因此，得出的结论是，含有纳米纤维的蜂蜜可以成为基于干细胞的伤口愈合和再生医学的有效基质。