The mechanism of nanoparticles (NPs) synthesis is a crucial prerequisite for the engineering of desirable material properties and is elusive due to the difficulty of studying the synthesis path and also due to lack of published work. The present study moves beyond the current conventional study of characterisation of NPs synthesis. It focuses on study of classical kinetics of metal NP using silver as a model metal to perform the required experiments, it aimed at understanding the crystallisation process which is the major root for the synthesis of metal NP. The authors have chosen biological approach to explain the process of synthesis of metal NP using inductively coupled plasma - optical emission spectroscopy that directly analyses the concentration of metal NPs. Alpha-amylase solution, when incubated with silver nitrate solution, turned brown at a specific concentration of enzyme and substrate, which shows the formation of silver NPs. Inductively coupled plasma data and dynamic light scattering spectra were studied to understand the kinetics and the kinetics model was obtained. The enthalpy (ΔH*), activation energy (ΔE*), and equilibrium constant (K) were calculated for understanding the thermodynamics of the reaction. The process of NP synthesis is dependent on the kinetics of the reaction, and other process parameters limit the thermodynamics of the process.

中文翻译：

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深入了解银纳米粒子的动力学和热力学控制生物合成。

纳米粒子 (NPs) 合成的机制是设计理想材料性能的关键先决条件，并且由于合成路径的研究困难以及缺乏已发表的工作而难以捉摸。本研究超越了目前对 NPs 合成表征的常规研究。它侧重于研究金属 NP 的经典动力学，使用银作为模型金属进行所需的实验，旨在了解作为金属 NP 合成的主要根源的结晶过程。作者选择了生物学方法来解释金属 NP 的合成过程，该过程使用直接分析金属 NPs 浓度的电感耦合等离子体-发射光谱。α-淀粉酶溶液，当与硝酸银溶液一起孵育时，在特定浓度的酶和底物下变成棕色，这表明银纳米粒子的形成。研究了电感耦合等离子体数据和动态光散射光谱以了解动力学并获得动力学模型。计算焓 (ΔH*)、活化能 (ΔE*) 和平衡常数 (K) 以了解反应的热力学。NP 合成的过程取决于反应的动力学，而其他工艺参数限制了该过程的热力学。计算平衡常数 (K) 以了解反应的热力学。NP 合成的过程取决于反应的动力学，而其他工艺参数限制了该过程的热力学。计算平衡常数 (K) 以了解反应的热力学。NP 合成的过程取决于反应的动力学，而其他工艺参数限制了该过程的热力学。