Scaffolds based on chitosan (CTS), collagen (Coll) and glycosaminoglycans (GAG) mixtures cross-linked by tannic acid (TA) with bioglass 45S5 addition were obtained with the use of the freeze-drying method. The prepared scaffolds were characterised for morphology, mechanical strength and degradation rate. Moreover, cell viability on the obtained scaffolds was measured with and without the presence of ascorbic acid and dexamethasone. The main purpose of the research was to compare the effectiveness of bioglass 45S5 influence on the physicochemical and biological properties of scaffolds. The results demonstrated that the scaffolds based on the blends of biopolymers cross-linked by TA are stable in an aqueous environment. Scanning electron microscope images allowed the observation of a porous scaffold structure with interconnected pores. The addition of bioglass nanoparticles improved the mechanical properties and decreased the degradation rate of composite materials. The biological properties were improved for 20% tannic acid addition compared to 5%. However, the addition of bioglass 45S5 did not change to cells response significantly.

中文翻译：

---

基于壳聚糖和胶原蛋白与生物玻璃 45S5 的支架的性能。

使用冷冻干燥法获得了基于壳聚糖 (CTS)、胶原蛋白 (Coll) 和糖胺聚糖 (GAG) 混合物通过单宁酸 (TA) 交联并添加生物玻璃 45S5 的支架。对制备的支架进行形态学、机械强度和降解率的表征。此外，在存在和不存在抗坏血酸和地塞米松的情况下测量获得的支架上的细胞活力。该研究的主要目的是比较生物玻璃45S5对支架理化和生物学特性的影响。结果表明，基于 TA 交联的生物聚合物混合物的支架在水性环境中是稳定的。扫描电子显微镜图像允许观察具有互连孔的多孔支架结构。生物玻璃纳米粒子的添加改善了复合材料的力学性能并降低了降解速率。与添加 5% 的单宁酸相比，添加 20% 的单宁酸改善了生物学特性。然而，添加生物玻璃 45S5 并没有显着改变细胞反应。