In this study, probiotic yeast Saccharomycopsis fibuligera (S. fibuligera) VIT-MN04 was encapsulated with wheat bran fibre (WBF) and exopolysaccharide (EPS) along with 5% polyvinylpyrrolidone (PVP) using electrospinning technique for easy gastrointestinal transit (GIT). The electrospinning materials viz. WBF (10%), EPS (15%), PVP (5%) and electrospinning parameters viz. applied voltage (10 kV) and tip to collector distance (15 cm) were optimised using response surface methodology to produce fine nanofibres to achieve maximum encapsulation efficiency (100%) and GIT tolerance (97%). The probiotic yeast was successfully encapsulated in nanofibre and investigated for potential properties. The survival of encapsulated S. fibuligera VIT-MN04 was increased compared to the free cells during in vitro digestion. In addition, encapsulated yeast cells retained their viability during storage at 4°C for 56 days. The nanofibres were characterised using scanning electron microscopy, atomic force microscopy, X-ray diffraction, thermogravimetric analysis, zeta potential analysis and Fourier transform infrared spectroscopy followed by gas chromatography-mass spectrometry and nuclear magnetic resonance analysis. This work provides an efficient approach for encapsulation of probiotic yeast with the nanofibres which can also broaden the application of the prebiotic like WBF providing an idea for the efficient preparation of functional synbiotic supplements in the food industry.

中文翻译：

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使用静电纺丝技术对 Saccharomycopsis fibuligera VIT-MN04 进行纳米封装，以方便胃肠道转运。

在这项研究中，益生菌酵母 Saccharomycopsis fibuligera (S. fibuligera) VIT-MN04 使用静电纺丝技术与麦麸纤维 (WBF) 和胞外多糖 (EPS) 以及 5% 聚乙烯吡咯烷酮 (PVP) 一起封装，以方便胃肠道运输 (GIT)。静电纺丝材料即。WBF (10%)、EPS (15%)、PVP (5%) 和静电纺丝参数即。使用响应面方法优化施加的电压（10 kV）和尖端到收集器的距离（15 cm）以生产细纳米纤维，以实现最大的封装效率（100%）和 GIT 容差（97%）。益生菌酵母被成功封装在纳米纤维中，并研究了其潜在特性。在体外消化过程中，与游离细胞相比，封装的 S. fibuligera VIT-MN04 的存活率增加。此外，封装的酵母细胞在 4°C 下储存 56 天时保持其活力。使用扫描电子显微镜、原子力显微镜、X 射线衍射、热重分析、zeta 电位分析和傅里叶变换红外光谱以及随后的气相色谱-质谱和核磁共振分析对纳米纤维进行了表征。这项工作提供了一种用纳米纤维包裹益生菌酵母的有效方法，这也可以拓宽像 WBF 这样的益生元的应用，为在食品工业中有效制备功能性合生元补充剂提供了思路。zeta 电位分析和傅里叶变换红外光谱，然后是气相色谱-质谱和核磁共振分析。这项工作提供了一种用纳米纤维包裹益生菌酵母的有效方法，这也可以拓宽像 WBF 这样的益生元的应用，为在食品工业中有效制备功能性合生元补充剂提供了思路。zeta 电位分析和傅里叶变换红外光谱，然后是气相色谱-质谱和核磁共振分析。这项工作提供了一种用纳米纤维包裹益生菌酵母的有效方法，这也可以拓宽像 WBF 这样的益生元的应用，为在食品工业中有效制备功能性合生元补充剂提供了思路。