The main obstacle to biopharmaceutical delivery in therapeutic concentration into the brain for treating neurological disorders is the presence of the Blood-Brain Barrier (BBB). The physiological process of Receptor-Mediated Transcytosis (RMT) to transport cargo through the brain endothelial cells toward brain parenchyma has prompted researchers to search for non-natural ligands that can be used to transport drugs across the BBB. Conjugation of drugs to RMT ligands would be an effective strategy for its delivery to the central nervous system. An attractive approach to identify novel transcytosing ligands is the screening by phage display combinatorial libraries. The main technology strength lies in the large variety of exogenous peptides or proteins displayed on the phage's surface. Here, we provide a mini-review of phage display technology using in vitro and in vivo BBB models for the development of peptide-mediated drug delivery systems.

将治疗浓度的生物药物输送到大脑以治疗神经系统疾病的主要障碍是血脑屏障 (BBB) 的存在。受体介导的胞吞作用 (RMT) 将货物通过脑内皮细胞运输到脑实质的生理过程促使研究人员寻找可用于跨 BBB 运输药物的非天然配体。药物与 RMT 配体的结合将是将其递送至中枢神经系统的有效策略。鉴定新型转胞吞配体的一种有吸引力的方法是通过噬菌体展示组合文库进行筛选。主要的技术优势在于在噬菌体表面展示的种类繁多的外源肽或蛋白质。在这里，