To investigate the effect of Polygonum multiflorum-Andrographis paniculata intercropping system on rhizosphere soil actinomycetes of P. multiflorum, the community structure and diversity of soil actinomycetes were studied by using the original soil as the control group and the rhizosphere soil actinomycetes communities of P. multiflorum under monoculture and intercropping systems as the experimental group. In this study 655 221 effective sequences were obtained with an average length of 408 bp. OTU coverage and rarefaction curve showed that the sequencing could represent the real situation of soil actinomycetes. According to the results of alpha diversity analysis, the diversity soil actinomycetes varied as follows: original soil>intercropping soil>monoculture soil. The soil actinomycetes community structure and the relative abundance of dominant genera were significantly changed by both monoculture and intercropping, especially monoculture. OTU clustering and PCA analysis of soil samples showed that all the soil samples were divided into three distinct groups and the original soil was more similar to intercropping soil. In addition, intercropping increased the relative abundance of some beneficial actinomyces, such as Kitasatospora and Mycobacterium, which was beneficial to maintain soil health and reduce the occurrence of soil-borne diseases. The results show that, P. multiflorum-A. paniculata intercropping reduced the change of community structure and the decrease of diversity of soil actinomycetes caused by P. multiflorum monoculture, and made the actinomycete community in rhizosphere soil of P. multiflorum close to the original soil.

中文翻译：

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何首乌-穿心莲间作系统对何首乌根际土壤放线菌群落结构和多样性的影响

为了研究何首乌-穿心莲间作系统对何首乌根际土壤放线菌的影响，以原始土壤为对照组，对何首乌根际土壤放线菌群落进行了研究。在单作和间作系统下作为实验组。在这项研究中，获得655 221条有效序列，平均长度为408 bp。OTU覆盖率和稀疏度曲线表明，该序列可以代表土壤放线菌的真实情况。根据α多样性分析的结果，土壤放线菌的多样性变化为：原始土壤>间作土壤>单一栽培土壤。单作和间作，尤其是单作，土壤放线菌的群落结构和优势属的相对丰度均发生了显着变化。OTU聚类和土壤样品的PCA分析表明，所有土壤样品均分为三个不同的组，原始土壤与间作土壤更相似。此外，间作增加了一些有益的放线菌的相对丰度，如北孢菌和分枝杆菌，这有利于保持土壤健康并减少土壤传播疾病的发生。结果表明，何首乌A。沙枣间作减少了何首乌单一培养引起的群落结构的变化和土壤放线菌多样性的降低，并使其根际土壤中的放线菌群落化。