Background: Lately, the employment of nano-carriers has been known as an optimistic means of drug and vaccine delivery.

Objective: Nano vaccines are a novel tier of vaccines that can develop humoral and cellular immune responses and can be introduced as a practical and secure nano vaccine candidate for the prevention of diseases. The purpose of this study was to accomplish and use biodegradable nano-carriers for the synthesis of pentavalent vaccine and immunogenicity evaluation in the animal models. The PLGA nanoparticle was prepared and modified with chitosan nanoparticles. Nano-carrier PLGA-chitosan was loaded by the DTP-HepB-Hib antigens and confirmed by DLS, SEM, TEM and FT-IR, then in vitro loading and release were evaluated. Toxicity was assessed by the MTT method in the Hec293 cells. Antigenicity evaluation and histopathological study were conducted by injection of new nano pentavalent vaccines in the BALB/c mice and the immune response was measured in the serum samples through an indirect ELISA method.

Results: Conclusions drawn from the current study exhibited the plausible ability of nano-carrier to deliver the vaccine. A notable increase was shown in total IgG and IgM antibodies examined in mice vaccinated with new nano vaccines. The histopathological study in the treated mice showed no toxicity in the vital organs of mice.

Conclusion: The engineered vaccine delivery system showed the ability to induce robust immune responses and also suitability features of the PLGA-chitosan as a promising carrier to improve vaccine delivery.

背景：最近，纳米载体的使用被认为是一种乐观的药物和疫苗递送方式。

目的：纳米疫苗是一种新型的疫苗，可以产生体液和细胞免疫反应，可以作为一种实用且安全的纳米疫苗候选物来预防疾病。本研究的目的是完成和使用可生物降解的纳米载体在动物模型中合成五价疫苗和免疫原性评价。PLGA 纳米颗粒是用壳聚糖纳米颗粒制备和改性的。纳米载体PLGA-壳聚糖负载DTP-HepB-Hib抗原并通过DLS、SEM、TEM和FT-IR确认，然后评估体外负载和释放。在 Hec293 细胞中通过 MTT 方法评估毒性。

结果：从当前研究得出的结论表明纳米载体具有递送疫苗的合理能力。在接种新纳米疫苗的小鼠中检测到的总 IgG 和 IgM 抗体显着增加。接受治疗的小鼠的组织病理学研究表明，小鼠的重要器官没有毒性。

结论：工程疫苗递送系统显示出诱导强大免疫反应的能力，以及 PLGA-壳聚糖作为改善疫苗递送的有前途的载体的适用性特征。