The metabolic engineering of carbon monoxide (CO) oxidizers has the potential to create efficient biocatalysts to produce hydrogen and other valuable chemicals. We herein applied markerless gene deletion to CO dehydrogenase/energy-converting hydrogenase (CODH/ECH) in the thermophilic facultative anaerobe, Parageobacillus thermoglucosidasius. We initially compared the transformation efficiency of two strains, NBRC 107763T and TG4. We then disrupted CODH, ECH, and both enzymes in NBRC 107763T. The characterization of growth in all three disruptants under 100% CO demonstrated that both enzymes were essential for CO-dependent growth with hydrogen production in P. thermoglucosidasius. The present results will become a platform for the further metabolic engineering of this organism.

中文翻译：

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嗜热葡萄糖副杆菌一氧化碳依赖制氢机制的基因工程

一氧化碳 (CO) 氧化剂的代谢工程有可能创造有效的生物催化剂来生产氢气和其他有价值的化学品。我们在此将无标记基因删除应用于嗜热兼性厌氧菌中的 CO 脱氢酶/能量转换氢化酶 (CODH/ECH)，副芽孢杆菌 Thermoglucosidasius。我们最初比较了两种菌株 NBRC 107763T 和 TG4 的转化效率。然后我们破坏了 NBRC 107763T 中的 CODH、ECH 和两种酶。在 100% CO 下所有三种破坏剂的生长特征表明，这两种酶对于 P. thermoglucosidasius 中产氢的 CO 依赖性生长至关重要。目前的结果将成为该生物进一步代谢工程的平台。