Abstract An advanced protocol to prepare single extant and fossil pollen grains for transmission electron microscopy (TEM) analysis allows for the fast recovery of data on the ultrastructure of pollen/spores. The protocol is easy to apply and less time consuming than previous methods. The ‘loss’ of pollen grains and pollen that is ‘difficult to locate’ within the embedding material is avoided, and each single pollen grain can be prepared successfully for TEM analysis. This preparation method is meant as an addition to the single-grain method using combined light and scanning electron microscopy to investigate dispersed fossil pollen grains developed by Dr Reinhard Zetter in the late 1980s.

中文翻译：

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单粒方法：将 TEM 添加到方程中

摘要：为透射电子显微镜 (TEM) 分析准备单个现存花粉粒和化石花粉粒的先进协议允许快速恢复花粉/孢子超微结构的数据。与以前的方法相比，该协议易于应用且耗时更少。避免了花粉粒的“丢失”和在包埋材料中“难以定位”的花粉，并且可以成功地制备每个花粉粒进行TEM分析。这种制备方法是对使用组合光学和扫描电子显微镜来研究由 Reinhard Zetter 博士在 1980 年代后期开发的分散的化石花粉颗粒的单颗粒方法的补充。