Abstract

A study of the effect of a seasonal decrease in ambient temperature on the composition of free amino acids and ninhydrin-positive secondary metabolites in gastrocnemius muscles of the frog Rana temporaria revealed that at the onset of winter anabiosis, as compared to the summer season, the alanine pool increased from 2.43 to 6.10 µmol/g w.w., and the 3-methylhistidine pool rose from 1.52 to 2.80 µmol/g w.w. For the first time, muscles of poikilothermic animals (namely, frogs) were found to contain cysteic acid and phosphoethanolamine, the level of which increased by the onset of hibernation, particularly for cysteic acid (from 0.91 to 2.15 µmol/g w.w.). The taurine level proved to be far lower compared to animals both of lower and higher phylogenetic ranks, but by the onset of hibernation it increased almost threefold. Presumably, the accumulation of the above metabolites relates to their protective role at temperatures that are extremely low for the survival of R. temporaria.

中文翻译：

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拟南芥初发时蛙林的腓肠肌的低温适应

摘要

研究环境温度的季节性降低对蛙蛙蛙腓肠肌游离氨基酸和茚三酮阳性次生代谢产物组成的影响揭示了与冬季相比，冬季再活化的开始，丙氨酸池从2.43增加到6.10 µmol / g湿重，而3-甲基组氨酸池从1.52到2.80 µmol / g湿重第一次，肌肉发现有60例Poikilothermic动物（即青蛙）中含有半胱氨酸和磷酸乙醇胺，尤其是对于半胱氨酸，其浓度随着冬眠的开始而增加（从0.91到2.15 µmol / g ww）。与较低和较高系统发育等级的动物相比，牛磺酸水平被证明要低得多，但是到冬眠开始时，牛磺酸水平几乎增加了三倍。据推测，上述代谢物的积累与其在温度下的生存能力极低的温度下的保护作用有关。