Reducing the reliance on hazardous agrochemicals (i.e., chemical fertilizers) that damage agro-ecosystems represents great challenges in modern agriculture. Sulfur-enriched biochar derived from organic wastes in the presence of sulfur-oxidizing bacteria could be a promising alternative to inorganic phosphorus fertilizers for sustainable P use in highly calcareous soils. This incubation experiment examined the impacts of applying a mixture of bone-wood chips biochar and sulfur at different rates with sulfur-oxidizing bacteria as well as incubation periods on availability and fraction changes of P in high P-fixing soil as calcareous sandy soil. In this study, 100 g air-dried soil was placed in plastic jars. It contains 3 treatments: control is unamended soil, soil amended with 0.25 g of biochar amendment jar⁻¹, and soil amended with 0.50 g of biochar amendment jar⁻¹. Soil in all treatments was inoculated by sulfur-oxidizing bacteria (Thiobacillus spp.). The results revealed that applying biochar amendment improved significantly phosphorus availability compared with control. Olsen-P increased with increasing incubating periods for bone char treatments and control. The value of Olsen-P was highest when 0.25 g and 0.50 g of biochar amendments jar⁻¹ were added (32 and 34 mg kg⁻¹, respectively) and the lowest in the control treatment (29 mg kg⁻¹). Applying biochar amendments to calcareous sandy soil significantly enhanced labile P and P associated with calcium fractions compared to control. Based on those obtained results, using sulfur-enriched biochar in calcareous sandy soil is beneficial because it can be a slow-release fertilizer, has a low cost, and is a clean alternative to mineral phosphate fertilizers.

减少对破坏农业生态系统的有害农用化学品（即化肥）的依赖是现代农业中的巨大挑战。在高钙质土壤中可持续使用磷，在存在硫氧化细菌的情况下，源自有机废物的富含硫的生物炭可能是无机磷肥料的有前途的替代品。在该温育实验研究施加骨木片生物碳硫在含有硫氧化细菌以及对P中的可用性和分数的变化的潜伏期不同速率的混合物和所产生的影响高磷-定影土壤如钙质砂质土壤。在这项研究中，将100克风干土壤放入塑料罐中。它包含3种处理方法：对照是未改​​良的土壤，用0.25 g的生物炭改良罐^-1改良过的土壤，以及用0.50 g的生物炭改良罐^-1改良过的土壤。在所有处理中，土壤均接种了硫氧化细菌（硫杆菌属）。结果表明，与对照相比，施用生物炭改良剂可显着提高磷的利用率。Olsen-P随骨炭处理和对照潜伏期的增加而增加。当添加0.25 g和0.50 g生物炭改良剂jar ^-1（32和34 mg kg ^-1时，Olsen-P的值最高）分别为）和最低（29 mg kg ^-1）。与对照相比，在钙质砂质土壤上施用生物炭改良剂显着增强了不稳定的P和与钙组分相关的P。根据获得的结果，在钙质砂质土壤中使用富含硫的生物炭是有益的，因为它可以是缓释肥料，成本低廉，是矿物磷酸盐肥料的清洁替代品。