Methylene Blue (MB) based Photodynamic therapy (PDT) has gained much interest recently due to its FDA approval for treating various diseased conditions in human. Herein, we report for the first time the systematic evaluation of NIR-controlled PDT efficiency of free MB and MB attached on NaYF₄:Yb, Er nanoparticles (UCNPs) i.e., MB@UCNPs encapsulated within Liposomes (LPs). The UCNPs act as an upconverting energy source for the photosensitizers dye MB for enhanced ROS generation and utilizing the remnant energy for selective tracking or bioimaging. The specificity of our nano LPs system was achieved by conjugating our newly discovered anti-HER2 peptides by phage display technique and demonstrated its specificity by confocal imaging of HER-2 overexpressing in vitro model. A significant decline in the cell viability by 83% and 64% was achieved by the LPs containing MB@UCNPs and free MB with UCNPs, respectively after 975 nm NIR laser exposure (0.60 W/cm⁻²) for 5 min continuous exposure. Also, the flowcytometry assay confirmed that enhanced ROS generation capability of LPs with MB@UCNPs was higher than the free MB with UCNPs, which states the relevance of the minimal distance between MB and UCNPs for higher anti-cancer efficiency.

基于亚甲蓝（MB）的光动力疗法（PDT）最近由于其FDA批准用于治疗人类各种疾病而倍受关注。在此，我们首次报告了对游离MB和附着在NaYF ₄：Yb，Er纳米颗粒（UCNPs）上的MB和MB的NIR控制的PDT效率的系统评价，即MB @ UCNPs包裹在脂质体（LPs）中。UCNP用作光敏剂染料MB的上转换能量源，以增强ROS的产生，并利用剩余能量进行选择性跟踪或生物成像。我们的纳米LPs系统的特异性是通过噬菌体展示技术结合我们新发现的抗HER2肽来实现的，并通过共聚焦成像体外过表达的HER-2证明了其特异性模型。由83％和64％的细胞存活率下降显著用含有MB @ UCNPs和免费MB与UCNPs，后分别975纳米NIR激光曝光（0.60瓦/厘米脂多糖实现^-2）5分钟连续暴露。而且，流式细胞术测定证实具有MB @ UCNP的LP增强的ROS产生能力高于具有UCNP的游离MB，这表明MB和UCNP之间的最小距离对于更高的抗癌效率具有相关性。