Sheathless microflow cytometer utilizing two bulk standing acoustic waves,Cytometry Part A

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Sheathless microflow cytometer utilizing two bulk standing acoustic waves
Cytometry Part A ( IF 2.5 ) Pub Date : 2021-05-06 , DOI: 10.1002/cyto.a.24362
Ce Wang _{1,

2} , Yuting Ma ₂ , Zhongxiang Chen ₂ , Yunliang Wu ₂ , Feifei Song ₂ , Jianping Qiu ₃ , Mengdie Shi ₃ , Xiaodong Wu _{1,

2}

Affiliation

In recent years, microflow cytometry has become a popular research field because of its potential to provide low-cost and disposable chips for complex cell analyses. Herein, we demonstrate a sheathless microflow cytometer which integrates a bulk standing acoustic wave based microchip capable of three dimensional cell focusing. Flow cytometry was successfully demonstrated using this system with a coefficient of variation (CV) of 2.16% with standard calibration beads. The sensitivities calibrated by rainbow beads are 518 MEFL in fluorescein Isothiocyanate (FITC) channel and 264 MEPE in P-phycoerythrin (PE) channels, respectively. The linearities are more than 99% in both channels. The capability of the proposed microflow cytometer is further demonstrated by immunologically labeled leukocytes differentiation in blood. This acoustic-based microflow cytometer did not require any sheath flows or complex structures and can be mass produced. Because of the simple fluid channel, the chip can be easily made pipeless, disposable for applications requiring no cross contamination. Moreover, with the gentle and bio-compatible acoustic waves used, this technique is expected to maintain the viability of cells and other bioparticles.

中文翻译：

利用两个体驻声波的无鞘微流式细胞仪

近年来，微流式细胞术因其为复杂细胞分析提供低成本和一次性芯片的潜力而成为一个流行的研究领域。在此，我们展示了一种无鞘微流式细胞仪，它集成了基于体声波的微芯片，能够进行三维细胞聚焦。使用该系统成功地展示了流式细胞术，其变异系数 (CV) 为 2.16%，使用标准校准珠。彩虹珠校准的灵敏度分别为异硫氰酸荧光素 (FITC) 通道中的 518 MEFL 和 P-藻红蛋白 (PE) 通道中的 264 MEPE。两个通道的线性度都超过 99%。血液中免疫标记的白细胞分化进一步证明了所提出的微流式细胞仪的能力。这种基于声学的微流式细胞仪不需要任何鞘流或复杂的结构，并且可以批量生产。由于简单的流体通道，芯片可以很容易地制成无管道、一次性的，用于不需要交叉污染的应用。此外，通过使用温和且生物相容的声波，该技术有望保持细胞和其他生物颗粒的活力。

更新日期：2021-05-06

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