Regulation of mRNA steady-state levels is important in controlling gene expression particularly in response to environmental stimuli. This allows cells to rapidly respond to environment changes. The highly conserved nonsense-mediated mRNA decay (NMD) pathway was initially identified as a pathway that degrades aberrant mRNAs. NMD is now recognized as a pathway with additional functions including precisely regulating the expression of select natural mRNAs. Majority of these natural mRNAs encode fully functional proteins. Regulation of natural mRNAs by NMD is activated by NMD targeting features and environmental cues. Here, we show that Saccharomyces cerevisiae strains from three genetic backgrounds respond differentially to NMD depending on the environmental stimuli. We found that wild type and NMD mutant W303a, BY4741, and RM11-1a yeast strains respond similarly to copper in the environment but respond differentially to toxic cadmium. Furthermore, the PCA1 alleles encoding different mRNAs from W303a and RM11-1a strains are regulated similarly by NMD in response to the bio-metal copper but differentially in response to toxic cadmium.

中文翻译：

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不同酿酒酵母遗传背景对金属离子和无义介导的 mRNA 衰变的反应变化

mRNA 稳态水平的调节对于控制基因表达非常重要，尤其是对环境刺激的反应。这使细胞能够快速响应环境变化。高度保守的无义介导的 mRNA 衰变 (NMD) 途径最初被确定为降解异常 mRNA 的途径。NMD 现在被认为是一种具有附加功能的途径，包括精确调节选定的天然 mRNA 的表达。大多数这些天然 mRNA 编码功能齐全的蛋白质。NMD 对天然 mRNA 的调节由 NMD 靶向特征和环境线索激活。在这里，我们展示了Saccharomyces cerevisiae根据环境刺激，来自三种遗传背景的菌株对 NMD 的反应不同。我们发现野生型和 NMD 突变体 W303a、BY4741 和 RM11-1a 酵母菌株对环境中的铜的反应相似，但对有毒镉的反应不同。此外，编码来自 W303a 和 RM11-1a 菌株的不同 mRNA 的PCA1等位基因在响应生物金属铜时受到 NMD 的类似调节，但对有毒镉的反应不同。