Amphibians find a suitable habitat in rice agroecosystems, but their viability is affected by the sublethal effects of agrochemicals. Animal condition, external morphology, and liver and male gonad tissue histology were examined on Lysapsus limellum and Rhinella bergi, common species that occurs in rice fields (RF) and reference sites (RS) from Santa Fe and Chaco provinces, Argentina, respectively. Animal condition factor showed no significant differences between environments in either species. Males of both species from RF showed asymmetrical testis size. A higher proportion of cysts with early spermatogenesis cell stages were observed in RF specimens than in RS individuals. The proportion of tubules with free spermatozoa differed between environments for L. limellum. Bidder organ presented vacuoles, atretic follicles, and pigmentary cells in R. bergi from Chaco RF. Liver tissues of both species from RF exhibited sinusoid enlargement, hypervascularization, hepatocyte vacuolization, and dilated blood vessels. R. bergi had a higher number of hepatic melanomacrophages in RF than in RS. The histological biomarker was more sensitive than the morphological one to evaluate chronic pesticide exposure using anuran non-target organs (liver, gonads) and Bidder organ. The potential effect of those alterations on the reproductive performance of anurans that commonly occur in agroecosystems needs further research.

两栖动物可在水稻农业生态系统中找到合适的栖息地，但其生存能力受到农用化学品的亚致死作用的影响。研究了Lysapsus limellum和Rhinella bergi的动物状况，外部形态以及肝和雄性生殖腺组织的组织学，这是常见的物种，分别存在于阿根廷圣菲和查科省的稻田（RF）和参考位点（RS）。动物条件因子在两种物种之间的环境之间均无显着差异。来自RF的两个物种的雄性都显示出不对称的睾丸大小。与RS个体相比，在RF标本中观察到具有早期精子形成细胞阶段的囊肿比例更高。石灰乳的不同环境中，具有游离精子的小管的比例有所不同。投标人器官来自Chaco RF的Berg。R. bergi中出现空泡，闭锁卵泡和色素细胞。来自RF的两种物种的肝组织均表现出正弦曲线增大，过度血管形成，肝细胞空泡化和血管扩张。R. bergi在RF中的肝黑色素巨噬细胞数量高于RS。组织学生物标志物比形态学标志物更灵敏，可以评估使用无核非靶器官（肝脏，性腺）和投标人器官的慢性农药暴露。这些改变对农业生态系统中普遍存在的无氧生殖能力的潜在影响有待进一步研究。