Cell Stem Cell ( IF 19.8 ) Pub Date : 2021-05-05 , DOI: 10.1016/j.stem.2021.04.031 Ayaka Yanagida 1 , Daniel Spindlow 1 , Jennifer Nichols 2 , Anish Dattani 1 , Austin Smith 1 , Ge Guo 1
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Human naive pluripotent cells can differentiate into extraembryonic trophectoderm and hypoblast. Here we describe a human embryo model (blastoid) generated by self-organization. Brief induction of trophectoderm leads to formation of blastocyst-like structures within 3 days. Blastoids are composed of three tissue layers displaying exclusive lineage markers, mimicking the natural blastocyst. Single-cell transcriptome analyses confirm segregation of trophectoderm, hypoblast, and epiblast with high fidelity to the human embryo. This versatile and scalable system provides a robust experimental model for human embryo research.
中文翻译：
幼稚干细胞囊胚模型捕获人类胚胎谱系分离
人类幼稚多能细胞可以分化为胚外滋养外胚层和下胚层。在这里，我们描述了通过自组织生成的人类胚胎模型（胚芽）。滋养外胚层的短暂诱导可在 3 天内形成囊胚样结构。胚泡由三个组织层组成，显示独特的谱系标记，模仿天然囊胚。单细胞转录组分析证实滋养外胚层、下胚层和外胚层的分离与人类胚胎高度保真。这种多功能且可扩展的系统为人类胚胎研究提供了强大的实验模型。
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