A 56 day feeding trial was conducted to study effects of different carbohydrate-to-lipid (CHO.L⁻¹) ratios on growth, serum biochemistry, intestinal digestion and hepatic metabolism of glucose and lipid in juvenile hybrid grouper (Epinephelus fuscoguttatus × ♂ E. lanceolatus). Results showed that moderate CHO.L⁻¹ ratio resulted in better growth performance (p < .05). In serum, glucose, insulin and alanine aminotransferase were enhanced as CHO.L⁻¹ ratios increased, whereas cholesterol was reduced (p < .05). Increased CHO.L⁻¹ ratios resulted in raised activity of amylase and reduced activity of lipase in gut (p < .05). Gene expression related to glucose transporters improved (p < .05). Enzyme activity and gene expression involved in hepatic glycolysis improved in a limited manner, whereas enzyme activity and gene expression related to hepatic gluconeogenesis and lipolysis were inhibited in high CHO.L⁻¹ ratio group. Enzyme activity of hepatic lipogenesis showed an elevated trend, including acetyl-CoA carboxylase and fatty acid synthase (p < .05). In conclusion, different dietary CHO.L⁻¹ ratios affected growth, serum biochemistry, intestinal digestion and hepatic metabolism of glucose and lipid in grouper. Moreover, juvenile grouper could utilize diet containing moderate CHO.L⁻¹ ratio.

中文翻译：

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膳食碳水化合物与脂质比例调节幼年杂交石斑鱼 (♀ Epinephelus fuscoguttatus × ♂ E. lanceolatus)：对生长、血清生化、肠道消化和肝脏葡萄糖和脂质代谢的影响

进行了一项为期 56 天的饲养试验，以研究不同碳水化合物与脂质 (CHO.L ^-1 ) 比率对杂种石斑鱼 ( Epinephelus fuscoguttatus × ♂ E ) 生长、血清生化、肠道消化和葡萄糖和脂质肝脏代谢的影响. 披针草）。结果表明，中等 CHO.L ^-1比率导致更好的生长性能 ( p  < .05)。在血清中，葡萄糖、胰岛素和丙氨酸氨基转移酶随着 CHO.L ^-1比率的增加而增强，而胆固醇则降低 ( p  < .05)。增加 CHO.L ^-1比率导致肠道中淀粉酶活性升高和脂肪酶活性降低 ( p  < .05)。与葡萄糖转运蛋白相关的基因表达得到改善（p  < .05）。与肝糖酵解有关的酶活性和基因表达以有限的方式改善，而与肝糖异生和脂肪分解相关的酶活性和基因表达在高 CHO.L ^-1比率组中受到抑制。肝脏脂肪生成的酶活性显示出升高的趋势，包括乙酰辅酶 A 羧化酶和脂肪酸合酶 ( p  < .05)。总之，不同的饮食 CHO.L ^-1比例影响石斑鱼的生长、血清生化、肠道消化和肝脏葡萄糖和脂质代谢。此外，幼石斑鱼可以利用含有中等CHO.L ^-1比率的饮食。