Toll-like receptors (TLRs) are components of innate immunity, but also have a role in carcinogenesis. The prognostic value of TLR5 and TLR8 tumor expression was examined in contrast with known risk markers Ki67 and p53. All HCC patients from Oulu University Hospital with available representative tumor sample were included in this study (n = 182). TLR5, TLR8, Ki67, and p53 expression were investigated by immunohistochemistry. The relation between patient survival and TLR, Ki67, and p53 expression was calculated with Cox regression adjusted for confounding factors. TLR5 cytoplasm intensity was associated with 5-year overall (strong 0.0% vs weak 23.4%, p < 0.001) and disease-specific (strong 0.0% vs weak 34.9%, p < 0.001) survival. TLR5 nuclei percentage was associated with poor 5-year disease-specific survival (high 16.3% vs low 31.5%, p = 0.022). In adjusted analysis, strong TLR5 cytoplasm intensity was an independent risk factor for poor 5-year overall (adjusted HR 1.88, 95% CI 1.26–2.81) and disease-specific (adjusted HR 2.00, 95% CI 1.27–3.15) survival. High Ki67 and p53 expression associated with 5-year overall- and disease-specific survival. TLR8 was not associated with patient survival. This study suggests that TLR5 expression is independently prognostic in HCC with similar point estimate as previously known p53.

中文翻译：

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肝细胞癌中的Toll样受体5和8

Toll 样受体 (TLR) 是先天免疫的组成部分，但也在致癌作用中起作用。与已知的风险标志物 Ki67 和 p53 相比，检查了 TLR5 和 TLR8 肿瘤表达的预后价值。本研究包括来自奥卢大学医院的所有具有代表性肿瘤样本的 HCC 患者（n = 182）。通过免疫组织化学研究 TLR5、TLR8、Ki67 和 p53 的表达。患者存活率与 TLR、Ki67 和 p53 表达之间的关系使用针对混杂因素调整的 Cox 回归计算。TLR5 细胞质强度与 5 年总体（强 0.0% 对弱 23.4%，p < 0.001）和疾病特异性（强 0.0% 对弱 34.9%，p < 0.001）存活率相关。TLR5 细胞核百分比与较差的 5 年疾病特异性生存率相关（高 16.3% 对低 31.5%，p = 0. 022)。在调整后的分析中，强 TLR5 细胞质强度是 5 年总体（调整后的 HR 1.88，95% CI 1.26-2.81）和疾病特异性（调整后的 HR 2.00，95% CI 1.27-3.15）生存率较差的独立危险因素。Ki67 和 p53 的高表达与 5 年总体和疾病特异性存活率相关。TLR8 与患者存活率无关。该研究表明 TLR5 表达在 HCC 中具有独立的预后作用，其点估计与先前已知的 p53 相似。