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Artificial Reproduction of Blue Bream (Ballerus ballerus L.) as a Conservative Method under Controlled Conditions
Animals ( IF 2.7 ) Pub Date : 2021-05-06 , DOI: 10.3390/ani11051326
Przemysław Piech , Roman Kujawa

The blue bream Ballerus ballerus (L.) is one of two species of the Ballerus genus occurring in Europe. The biotechnology for its reproduction under controlled conditions needs to be developed to conserve its local populations. Therefore, a range of experiments were performed to obtain valuable gametes from blue bream following hormonal stimulation with carp pituitary homogenate (CPH), Ovopel and Ovaprim. CPH and Ovopel were injected twice and Ovaprim—once, under a pectoral fin. The spawners were inspected 12 h after the last injection, and the gametes were collected. Eggs were collected from spawning females and fertilized with sperm from three males. The smallest number of ovulating blue bream (four females) was obtained from individuals stimulated with Ovaprim. There were six to nine ovulating females in the other groups, accounting for 60–90% of the females in the respective groups. The latency period was the shortest in the spawners stimulated with Ovopel. The fish in this group ovulated 14 h after the hormonal injection. Spawning was synchronized and all females spawned simultaneously. The highest average weight of eggs per fish (75.4 g) was obtained from the females stimulated with Ovopel, whereas the individuals stimulated with Ovaprim provided the smallest average amount of eggs (49.5 g). The study showed that blue bream spawners are not very sensitive to reproduction-related handling under controlled conditions.

中文翻译:

受控条件下人工繁殖蓝鲷(Ballerus ballerus L.)作为一种保守方法

蓝鲷Ballerus ballerus(L.)是Ballerus的两种物种之一属发生在欧洲。需要开发在受控条件下繁殖的生物技术,以保护其当地人口。因此,在用鲤鱼垂体匀浆(CPH),Ovopel和Ovaprim刺激荷尔蒙后,进行了一系列实验,从蓝鲷中获得了有价值的配子。将CPH和Ovopel注射两次,并在胸鳍下一次注射Ovaprim。最后一次注射后12小时检查产卵者,并收集配子。从产卵雌性中收集卵,并用三只雄性中的精子受精。从Ovaprim刺激的个体中获得最少数量的排卵蓝鲷(四只雌性)。其他组中有6至9个排卵女性,占各组女性的60–90%。在由Ovopel刺激的产卵器中,潜伏期最短。荷尔蒙注射后14小时,该组鱼排卵。产卵同步,所有雌性同时产卵。从Ovopel刺激的雌性中获得每条鱼卵的平均重量最高(75.4 g),而由Ovaprim刺激的个体提供的卵平均量最小(49.5 g)。研究表明,蓝鲷产卵器在受控条件下对繁殖相关的处理不是很敏感。而受Ovaprim刺激的个体提供的卵平均量最小(49.5 g)。研究表明,蓝鲷产卵器在受控条件下对繁殖相关的处理不是很敏感。而受Ovaprim刺激的个体提供的卵平均量最小(49.5 g)。研究表明,蓝鲷产卵器在受控条件下对繁殖相关的处理不是很敏感。
更新日期:2021-05-06
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