Heat shock transcription factors (HSFs) are critical regulators of plant responses to various abiotic and biotic stresses, including high temperature stress. HSFs are involved in regulating the expression of heat shock proteins (HSPs) by binding with heat stress elements (HSEs) to defend against high-temperature stress. The H. perforatum genome was recently fully sequenced; this provides a valuable resource for genetic and functional analysis. In this study, 23 putative HpHSF genes were identified and divided into three groups (A, B, and C) based on phylogeny and structural features. Gene structure and conserved motif analyses were performed on HpHSFs members; the DNA-binding domain (DBD), hydrophobic heptad repeat (HR-A/B), and exon-intron boundaries exhibited specific phylogenetic relationships. In addition, the presence of various cis-acting elements in the promoter regions of HpHSFs underscored their regulatory function in abiotic stress responses. RT-qPCR analyses showed that most HpHSF genes were expressed in response to heat conditions, suggesting that HpHSFs play potential roles in the heat stress resistance pathway. Our findings are advantageous for the analysis and research of the function of HpHSFs in high temperature stress tolerance in H. perforatum.

中文翻译：

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贯叶连翘中HSF基因家族的全基因组鉴定，分类和表达谱分析

热激转录因子（HSF）是植物对各种非生物和生物胁迫（包括高温胁迫）的反应的关键调节剂。HSF通过与热应激元件（HSE）结合来调节热激蛋白（HSP）的表达，从而防御高温胁迫。贯叶连翘基因组最近已完全测序。这为遗传和功能分析提供了宝贵的资源。在这项研究中，根据系统发育和结构特征，确定了23个假定的HpHSF基因，并将其分为三组（A，B和C）。对HpHSFs成员进行基因结构和保守基序分析；DNA结合结构域（DBD），疏水性七肽重复序列（HR-A / B）和外显子-内含子边界表现出特定的系统发生关系。此外，HpHSFs启动子区域中各种顺式作用元件的存在突显了它们在非生物胁迫响应中的调节功能。RT-qPCR分析表明，大多数HpHSF基因是在热条件下表达的，这表明HpHSF在热应激抗性途径中起潜在作用。我们的发现有利于分析和研究HpHSFs在贯叶连翘的高温胁迫耐受性中的功能。