To investigate whether activating the Wnt/β-catenin signalling pathway differentially promotes fracture healing in aged and adult individuals. Catnb^TM2Kem, Catnb^lox(ex3) and wild-type adult and aged mice were used in this study. The femur was electroporated through a hole with a diameter of 0.6 mm. On the 7th, 14th and 21st days after fracture establishment, repair of the femoral diaphyseal bone was examined using X-ray and CT, the levels of mRNAs related to Wnt/β-catenin signalling were detected using real-time polymerase chain reaction (RT-PCR), and angiogenesis and cell differentiation were observed using immunohistochemistry. The numbers of osteoclasts were determined by TRAP staining. Wnt/β-catenin activation accelerated fracture healing in adult mice, with more pronounced effects on aged mice. Compared with wild-type mice at the corresponding ages, Wnt/β-catenin signalling activation induced higher levels of angiogenesis and cell differentiation in aged mice than in adult mice and promoted fracture healing. The administration of medications targeting Wnt/β-catenin signalling to aged patients may accelerate fracture healing to a greater extent.

研究激活 Wnt/β-catenin 信号通路是否有差异地促进老年人和成人的骨折愈合。本研究使用Catnb ^TM2Kem、 Catnb ^{lox(ex3)以及野生型成年和老年小鼠。}通过直径为0.6mm的孔对股骨进行电穿孔。骨折建立后第7、14、21天，采用X线和CT检查股骨干骨修复情况，采用实时聚合酶链反应（RT）检测Wnt/β-catenin信号相关mRNA的水平。 -PCR），并使用免疫组织化学观察血管生成和细胞分化。通过TRAP染色测定破骨细胞的数量。Wnt/β-catenin 激活可加速成年小鼠的骨折愈合，对老年小鼠的影响更为明显。与相应年龄的野生型小鼠相比，Wnt/β-catenin信号激活诱导老年小鼠比成年小鼠更高水平的血管生成和细胞分化，并促进骨折愈合。对老年患者施用针对 Wnt/β-连环蛋白信号传导的药物可能会在更大程度上加速骨折愈合。