Background

Motor recovery after stroke in humans and in rodent models is time sensitive. Recovery in patients is a result of biological spontaneous recovery via endogenous repair mechanisms and is likely improved by enhancing the synaptic plasticity required for endogenous repair. Cerebrolysin is a polypeptide preparation known to enhance neuroplasticity and may improve recovery in patients. In mice, we tested the hypothesis that Cerebrolysin can act poststroke to enhance both spontaneous and training-associated motor recovery.

Methods

Mice were trained to perform a skilled prehension task. We then induced a photothrombotic stroke in the caudal forelimb area, after which we retrained animals on the prehension task in the presence or absence of Cerebrolysin after a 2-day or 8-day delay. Mice received daily intraperitoneal Cerebrolysin or saline injections starting poststroke day 1 or poststroke day 7.

Results

Prior studies showed that poststroke recovery of prehension can occur if animals receive rehabilitative training during an early sensitive period but is incomplete if rehabilitative training is delayed. In contrast, we show complete recovery of prehension, despite a delay in rehabilitative training, when mice receive daily Cerebrolysin administration starting on poststroke day 1 or on poststroke day 8. When Cerebrolysin is given on poststroke day 1, recovery occurred even in the absence of training. Stroke volumes were similar across groups.

Conclusions

Poststroke Cerebrolysin administration leads to recovery of motor function independent of rehabilitative training without a protective effect on stroke volume. This is one of the first demonstrations of training-independent motor recovery in rodent stroke models.

中文翻译：

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用 Cerebrolysin 治疗的小鼠中风后增强的自发运动恢复

背景

人类和啮齿动物模型中风后的运动恢复是时间敏感的。患者的恢复是通过内源性修复机制进行的生物自发恢复的结果，并且可能通过增强内源性修复所需的突触可塑性来改善。Cerebrolysin 是一种多肽制剂，已知可增强神经可塑性并可能改善患者的康复。在小鼠中，我们测试了 Cerebrolysin 可以在中风后发挥作用以增强自发和训练相关运动恢复的假设。

方法

训练小鼠执行熟练的抓握任务。然后，我们在前肢尾部区域诱导了光血栓形成中风，之后我们在延迟 2 天或 8 天后，在存在或不存在 Cerebrolysin 的情况下对动物进行了抓握任务的再训练。从中风后第 1 天或中风后第 7 天开始，小鼠每天接受腹膜内 Cerebrolysin 或盐水注射。

结果

先前的研究表明，如果动物在早期敏感期接受康复训练，则可以在中风后恢复抓握力，但如果延迟康复训练，则不完全。相比之下，当小鼠在中风后第 1 天或中风后第 8 天开始接受每日 Cerebrolysin 给药时，尽管康复训练有所延迟，但我们显示抓握力完全恢复。当在中风后第 1 天给予 Cerebrolysin 时，即使没有训练。各组的卒中量相似。

结论

中风后脑活素给药导致运动功能恢复独立于康复训练，对每搏输出量没有保护作用。这是啮齿动物中风模型中与训练无关的运动恢复的首次演示之一。