Background: Practitioners of traditional medicine use the decoction of Ononis natrix L. to treat hyperglycemia. The literature offers no evidence to support the use.

Objective: To investigate the effect of the decoction of Ononis natrix L. on the blood glucose concentration in Wistar rats (Rattus norvegicus) with streptozotocin-induced diabetes mellitus.

Methods: We obtained 35 Wistar rats from the animal colony of The University of Jordan School of Medicine. We induced diabetes by a single intraperitoneal injection of streptozotocin (60 mg/kg body weight) and 23 rats (66%) survived to allocation. We randomly assigned the rats to one of four groups: negative control (1% Tween 80 in distilled water), positive control (100 mg/kg metformin), high-dose treatment (7.5 mL of the decoction), and low-dose treatment (3.5 mL of the decoction). We administered the doses twice daily by oral gavage for two weeks and measured the tailblood glucose concentration twice daily, once before the first dose and another time after the second dose. We used linear mixed-effects regression to model the change in blood glucose concentration as a function of the experimentation groups, with adjustments for pseudoreplication and temporal variation.

Results: The estimated mean change was 1 mmol/L (−30 to 31 mmol/L) for the negative control group, −26 mmol/L (−56 to 5 mmol/L) for the positive control group, −75 mmol/L (−108 to −42) for the low-dose treatment group, and −82 mmol/L (−111 to −53 mmol/L) for the high-dose treatment group.

Conclusion: In conclusion, we demonstrate, for the first time, the hypoglycemic effect of Ononis natrix L. in an animal model of diabetes.

背景：传统医学的从业者使用Ononis natrix L.汤来治疗高血糖症。文献没有提供证据支持使用。

目的：探讨Ononis natrix L.汤对链脲佐菌素致糖尿病的Wistar大鼠（Rattus norvegicus）血糖浓度的影响。

方法：我们从约旦大学医学院的动物群落中获得了35只Wistar大鼠。我们通过单次腹膜内注射链脲佐菌素（60 mg / kg体重）诱导了糖尿病，并且有23只大鼠（66％）幸免于难。我们将大鼠随机分为四组之一：阴性对照组（蒸馏水中1％吐温80），阳性对照组（100 mg / kg二甲双胍），高剂量治疗（7.5 mL煎剂）和低剂量治疗（3.5mL的汤）。我们每天两次通过口管法给予剂量，持续两周，并每天两次测定尾血葡萄糖浓度，一次在第一次给药之前，另一次在第二次给药之后。我们使用线性混合效应回归模型来模拟血糖浓度随实验组的变化，

结果：阴性对照组的估计平均变化为1 mmol / L（−30至31 mmol / L），阳性对照组的为-26 mmol / L（−56至5 mmol / L），− 75 mmol / L低剂量治疗组为L（-108至-42），高剂量治疗组为-82 mmol / L（-111至-53 mmol / L）。

结论：总之，我们首次证明了Ononis natrix L.在糖尿病动物模型中的降血糖作用。