Abstract

Changes in the number of anaerobic microorganisms (denitrifying, sulfate, and iron-reducing) in ground and groundwater polluted with oil products have been investigated during bioremediation. It is shown that, in the studied geological media, the number of psychrophilic microorganisms of anaerobic groups (denitrifying and sulfate and iron-reducing, as assessed by the number of the colony-forming units on the respective cultivation media) is higher than the number of mesophilic ones. When N, P, and K fertilizers are introduced into the contaminated zone, the number of psychrophilic microorganisms changes more significantly, both increasing and decreasing. When the ground in the aeration zone is treated, the number of anaerobes decreases, probably due to competitive displacement by aerobes. The number of hydrocarbon-oxidizing and ammonifying microorganisms increases by an order of magnitude to 10⁶ CFU/g and 10⁶–10⁷ CFU/g, respectively. When fertilizers are added to groundwater, the number of anaerobic microorganisms increases 1-4 orders of magnitude and the number of denitrifiers reaches 10⁵ CFU/mL, whereas the CFU number of sulfate and iron reducers is 10³–10⁴ and 10²–10³ CFU/mL, respectively. It is revealed that, simultaneously with the increase in CFU numbers, some chemical properties change, indicating the intensified biodegradation of petroleum products. In particular, antiphase changes in the ammonium and nitrate concentrations are observed due to the decomposition of petroleum products, nitrification, and denitrification, as well as the concentration of bicarbonate and sulfate ions due to sulfate reduction.

中文翻译：

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刺激土著厌氧微生物对石油产品污染的地质环境的生物修复

摘要

在生物修复过程中，已经研究了被石油产品污染的地下水和地下水中厌氧微生物（脱硝，硫酸盐和还原铁）数量的变化。结果表明，在所研究的地质介质中，厌氧基团的嗜冷微生物的数量（通过相应培养基上菌落形成单位的数量评估为脱氮，硫酸盐和还原铁的数量）高于该数量。中温的。当氮，磷和钾肥被引入污染区时，嗜冷微生物的数量变化更为明显，既增加又减少。当对曝气区的地面进行处理时，厌氧菌的数量会减少，这可能是由于需氧菌竞争性置换所致。⁶ CFU / g和10 ⁶ –10 ⁷ CFU / g。在地下水中添加肥料时，厌氧微生物的数量增加1-4个数量级，反硝化剂的数量达到10 ⁵ CFU / mL，而硫酸盐和铁的还原剂的CFU数量为10 ³ –10 ⁴和10 ² – 10 ³分别为CFU / mL。结果表明，随着CFU数量的增加，一些化学性质发生变化，表明石油产品的生物降解加剧。特别是，由于石油产品的分解，硝化和反硝化，以及由于硫酸盐还原引起的碳酸氢根和硫酸根离子的浓度，观察到了铵和硝酸盐浓度的反相变化。