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Andrographolide production and enhanced antioxidant activity in Andrographis paniculata (Burm f.) Nees. promoted by seaweed liquid extracts
Brazilian Journal of Botany ( IF 1.6 ) Pub Date : 2021-05-05 , DOI: 10.1007/s40415-021-00715-x
Veeranan Uthirapandi , Sivasangari Ramya Subramanian , Boomibalagan Ponnerulan , Eswaran Saminathan , Vijayanand Narayanan , Kathiresan Durairaj

Andrographolide is important specialized metabolites of Andrographis paniculata (Acanthaceae) which is used to treat wide array of disorders such as cancer, diabetes, rheumatoid arthritis. An attempt was made to examine the potential of seaweed (Sargassum wightii Greville) on growth, andrographolide content and antioxidant activity in A. paniculata. Different concentrations (5%, 10%, 15% and 20%) of seaweed liquid extract (SLE) were applied through foliar application to A. paniculata (Burm. f.) Nees seedlings. Among the various concentrations, 10% of SLE exhibited a maximum increase in growth parameters such as plant height (113%), leaf surface area (129%), number of leaves and branch by 134% and 118%, fresh weight (178%) and dry weight (220%). Similarly, biochemical constituents such as starch (132%), glucose (122%), protein (177%) and total chlorophyll (213%) were also found to be enhanced in leaves of those plants which received 10% of SLE. A twofold (34.92 mg g−1) increased synthesis of andrographolide was noticed in 10% methanolic SLE treated plants. Similarly, antioxidant activity was increased in 10% methanolic SLE which accounted for 53.94 μg/ml DPPH activity and 44.94% inhibition of nitric oxide activity. The present study suggested that SLE of S. wightii could be a promising biostimulant which can be attributed to the presence of growth hormones like cytokinin (3.9 mg L−1), auxin (3.0 mg L−1) and gibberellin (2.2 mg L−1) and nutrients such as Na (45.47 mg L−1), Mg (75.12 mg L−1), P (4.03 mg L−1), K (58.4 mg L−1), Ca (0.432 mg L−1), Fe (0.85 mg L−1), Cu (0.346 mg L−1), Mb (0.0004 mg L−1) and N (2.56%) and also pave the way for sustainable environment approach in herbal cultivation.



中文翻译:

穿心莲中穿心莲内酯的产生和增强的抗氧化活性。由海藻液体提取物促进

穿心莲内酯是重要的代谢物专门穿心莲 其用于治疗诸如癌症,糖尿病,类风湿性关节炎的宽阵列(爵床科)。试图检查紫菜(Sargassum wightii Greville)潜在的生长,穿心莲内酯含量和抗氧化活性通过叶面施肥将不同浓度(5%,10%,15%和20%)的海藻液体提取物(SLE)施用到A. paniculata(Burm。f。)Nees幼苗。在各种浓度中,10%的SLE表现出最大的生长参数增加,例如株高(113%),叶表面积(129%),叶和枝数分别增加134%和118%,鲜重(178%)。 )和干重(220%)。同样,在接受了10%SLE的那些植物的叶子中,还发现诸如淀粉(132%),葡萄糖(122%),蛋白质(177%)和总叶绿素(213%)的生化成分得到增强。在10%甲醇SLE处理过的植物中,发现穿心莲内酯的合成增加了两倍(34.92 mg g -1)。同样,在10%的甲醇SLE中抗氧化剂活性增加,这导致53.94μg/ ml的DPPH活性和一氧化氮活性的抑制作用为44.94%。本研究表明,怀氏链球菌的系统性红斑狼疮可能是一种有前途的生物刺激剂,可以归因于生长激素的存在,例如细胞分裂素(3.9 mg L -1),生长素(3.0 mg L -1)和赤霉素(2.2 mg L -1)和诸如Na(45.47 mg L -1),Mg(75.12 mg L -1),P(4.03 mg L -1),K(58.4 mg L -1),Ca(0.432 mg L -1),Fe(0.85 mg L -1), Cu(0.346 mg L -1),Mb(0.0004 mg L -1)和N(2.56%),也为草药种植中的可持续环境方法铺平了道路。

更新日期:2021-05-06
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