Characteristics of biofilm-induced degradation at resin–dentin interfaces using multiple combinations of adhesives and resins,Dental Materials

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Characteristics of biofilm-induced degradation at resin–dentin interfaces using multiple combinations of adhesives and resins
Dental Materials ( IF 4.6 ) Pub Date : 2021-05-06 , DOI: 10.1016/j.dental.2021.04.007
Yuan Zhou ₁ , Khairul Matin ₂ , Yasushi Shimada ₃ , Alireza Sadr ₄ , Guoqing Wang ₅ , Junji Tagami ₆ , Xiping Feng ₁

Affiliation

Laboratory of Molecular and Preventive Dentistry, Department of Preventive Dentistry, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, Huangpu District, Shanghai 200011, China.
Department of Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan; Endowed Department of International Oral Health Science (affiliated with Department of Translational Research), School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama-shi, Kanagawa 230-0063, Japan.
Department of Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan; Department of Operative Dentistry, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8525, Japan.
Biomimetics Biomaterials Biophotonics & Technology Laboratory, Department of Restorative Dentistry, University of Washington School of Dentistry, 1959 NE Pacific Street, Seattle, WA 98195-7456, USA.
Department of Electrical and Electronic Engineering, Southern University of Science and Technology, 1088 Xueyuan Ave, Nanshan District, Shenzhen, Guangdong 518055, China.
Department of Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.

Objective

We aimed to evaluate morphological, mechanical and chemical characteristics at resin–dentin interfaces using multiple combinations of adhesives and resins after a short-term biofilm-induced degradation.

Methods

Cervical cavities were prepared in bovine incisors, treated by Clearfil SE Bond 2 (SE) or FL-Bond II (FL), restored by Clearfil Majesty ES Flow (ES) or Beautifil Flow Plus (BFP) and grouped into SE-ES, SE-BFP, FL-ES and FL-BFP. After biofilm challenge, interfacial gaps and dentin wall lesions were examined by optical coherence tomography (OCT). Gap depth (GD), gap pattern scale (GPS) and dentin wall lesion depth (WLD) were evaluated from confocal laser scanning microscope. Microhardness of dentin lesions was measured with a Vickers microhardness tester. Chemical elements in resins and dentin wall lesions were analyzed by scanning electron microscopy and energy dispersive X-ray spectrometry (SEM/EDS). Morphological structures of interfacial gaps were observed by SEM.

Results

OCT could detect adhesive-dentin-bonded and adhesive-dentin-debonded gaps. SE-containing groups showed significantly lower GPS than FL-containing groups. FL-BFP showed significantly lower WLD than FL-ES. Microhardness of dentin wall lesions was higher than that of outer lesions and they showed significant differences in FL-BFP. SE-BFP showed a lower GPS curve and higher intensities of Ca and P in the upper half of dentin wall lesions than other groups. From SEM, microgaps between filler and matrix, break and loss of matrix, separation of adhesive matrix with hybrid layer occurred at interfacial gaps.

Significance

The morphological, mechanical and chemical characteristics of resin–dentin interfacial degradation depend on the component and chemistry of restorative materials.

中文翻译：

使用粘合剂和树脂的多种组合在树脂-牙本质界面处生物膜诱导的降解特性

客观的

我们的目的是在短期生物膜诱导降解后，使用粘合剂和树脂的多种组合来评估树脂-牙本质界面的形态、机械和化学特性。

方法

用牛门牙制备颈腔，用 Clearfil SE Bond 2 (SE) 或 FL-Bond II (FL) 处理，用 Clearfil Majesty ES Flow (ES) 或 Beautifil Flow Plus (BFP) 修复，并分为 SE-ES、SE -BFP、FL-ES 和 FL-BFP。生物膜攻击后，通过光学相干断层扫描（OCT）检查界面间隙和牙本质壁病变。从共聚焦激光扫描显微镜评估间隙深度（GD）、间隙模式尺度（GPS）和牙本质壁病变深度（WLD）。用维氏显微硬度计测量牙本质损伤的显微硬度。通过扫描电子显微镜和能量色散 X 射线光谱法 (SEM/EDS) 分析树脂和牙本质壁病变中的化学元素。通过扫描电镜观察界面间隙的形态结构。

结果

OCT 可以检测粘着牙本质粘合和粘着牙本质脱粘的间隙。含 SE 组的 GPS 显着低于含 FL 组。FL-BFP 的 WLD 显着低于 FL-ES。牙本质壁病灶显微硬度高于外病灶，FL-BFP差异显着。SE-BFP 在牙本质壁病变的上半部分显示出较低的 GPS 曲线和较高的 Ca 和 P 强度。从扫描电镜来看，填料和基体之间的微间隙、基体的断裂和损失、粘合剂基体与混合层的分离发生在界面间隙处。