Solution NMR in human embryo culture media as an option for assessment of embryo implantation potential,NMR in Biomedicine

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Solution NMR in human embryo culture media as an option for assessment of embryo implantation potential
NMR in Biomedicine ( IF 2.7 ) Pub Date : 2021-05-06 , DOI: 10.1002/nbm.4536
Ana M Salmerón ₁ , Ana Cristina Abreu ₁ , Miguel Vilches-Ferrón ₂ , Ignacio Fernández ₁

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NMR offers the potential to holistically screen hundreds of metabolites and has already proved to be a powerful technique able to provide a global picture of metabolic changes in a wide range of biological systems underlying complex and multifactorial matrixes. This review covers the literature until May 2020 centered on the early prediction of the viability of in vitro developed embryos using several analytical techniques, including NMR. Nowadays, the predominant non-invasive technique for selecting viable embryos is based on morphology, where variables associated with the rate of cleavage and blastocyst formation are evaluated by the embryologist following standardized criteria that are somewhat subjective. This morphological approach is therefore inadequate for the prediction of embryo quality, and several studies have focused on developing new non-invasive methods using molecular approaches based particularly on metabolomics. This review outlines the potential of NMR as one of these non-invasive in vitro methods based on the analysis of spent embryo culture media.

中文翻译：

人类胚胎培养基中的溶液 NMR 作为评估胚胎植入潜力的一种选择

NMR 提供了全面筛选数百种代谢物的潜力，并且已被证明是一种强大的技术，能够提供复杂和多因素基质下的广泛生物系统中代谢变化的全球图景。这篇综述涵盖了截至 2020 年 5 月的文献，重点是体外可行性的早期预测使用包括核磁共振在内的多种分析技术开发了胚胎。如今，用于选择活胚胎的主要非侵入性技术是基于形态学的，其中与卵裂和囊胚形成率相关的变量由胚胎学家按照有点主观的标准化标准进行评估。因此，这种形态学方法不足以预测胚胎质量，并且有几项研究集中在使用特别基于代谢组学的分子方法开发新的非侵入性方法。本综述概述了核磁共振作为这些非侵入性体外方法之一的潜力，基于对用过的胚胎培养基的分析。

更新日期：2021-07-02

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