BRASSINOSTEROID-SIGNALLING KINASES 7 and 8 associate with the FLS2 immune receptor and are required for flg22-induced PTI responses,Molecular Plant Pathology

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BRASSINOSTEROID-SIGNALLING KINASES 7 and 8 associate with the FLS2 immune receptor and are required for flg22-induced PTI responses
Molecular Plant Pathology ( IF 4.8 ) Pub Date : 2021-05-06 , DOI: 10.1111/mpp.13062
Bharat Bhusan Majhi ₁ , Guy Sobol ₁ , Sarah Gachie ₁ , Shivakumar Sreeramulu ₁ , Guido Sessa ₁

Affiliation

Pattern-triggered immunity (PTI) is typically initiated in plants by recognition of pathogen- or damage-associated molecular patterns (PAMP/DAMPs) by cell surface-localized pattern recognition receptors (PRRs). Here, we investigated the role in PTI of Arabidopsis thaliana brassinosteroid-signalling kinases 7 and 8 (BSK7 and BSK8), which are members of the receptor-like cytoplasmic kinase subfamily XII. BSK7 and BSK8 localized to the plant cell periphery and interacted in yeast and in planta with FLS2, but not with other PRRs. Consistent with a role in FLS2 signalling, bsk7 and bsk8 single and bsk7,8 double mutant plants were impaired in several immune responses induced by flg22, but not by other PAMP/DAMPs. These included resistance to Pseudomonas syringae and Botrytis cinerea, reactive oxygen species accumulation, callose deposition at the cell wall, and expression of the defence-related gene PR1, but not activation of MAP kinases and expression of the FRK1 and WRKY29 genes. bsk7, bsk8, and bsk7,8 plants also displayed enhanced susceptibility to P. syringae and B. cinerea. Finally, BSK7 and BSK8 variants mutated in their myristoylation site or in the ATP-binding site failed to complement defective phenotypes of the corresponding mutants, suggesting that localization to the cell periphery and kinase activity are critical for BSK7 and BSK8 functions. Together, these findings demonstrate that BSK7 and BSK8 play a role in PTI initiated by recognition of flg22 by interacting with the FLS2 immune receptor.

中文翻译：

BRASSINOSTEROID 信号激酶 7 和 8 与 FLS2 免疫受体相关，是 flg22 诱导的 PTI 反应所必需的

模式触发免疫 (PTI) 通常在植物中通过细胞表面定位模式识别受体 (PRR) 识别病原体或损伤相关分子模式 (PAMP/DAMP) 来启动。在这里，我们研究了拟南芥油菜素类固醇信号激酶 7 和 8（BSK7 和 BSK8）在 PTI 中的作用，它们是受体样细胞质激酶亚家族 XII 的成员。BSK7 和 BSK8 定位于植物细胞外围，并在酵母和植物中与 FLS2 相互作用，但不与其他 PRR 相互作用。与 FLS2 信令中的作用一致，bsk7和bsk8单和bsk7,8双突变植物在 flg22 诱导的几种免疫反应中受损，但其他 PAMP/DAMPs 没有。这些包括对丁香假单胞菌和灰葡萄孢菌的抗性、活性氧物质积累、细胞壁胼胝质沉积和防御相关基因PR1 的表达，但不包括 MAP 激酶的激活和FRK1和WRKY29基因的表达。bsk7、bsk8和bsk7,8植物也表现出对丁香假单胞菌和B. cinerea增强的易感性. 最后，在肉豆蔻酰化位点或 ATP 结合位点突变的 BSK7 和 BSK8 变体未能补充相应突变体的缺陷表型，这表明定位到细胞外周和激酶活性对 BSK7 和 BSK8 功能至关重要。总之，这些发现表明 BSK7 和 BSK8 在通过与 FLS2 免疫受体相互作用识别 flg22 启动的 PTI 中发挥作用。

更新日期：2021-06-28

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