The Beta vulgaris-derived resistance gene Rz2 confers broad-spectrum resistance against soilborne sugar beet-infecting viruses from different families by recognizing triple gene block protein 1,Molecular Plant Pathology

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The Beta vulgaris-derived resistance gene Rz2 confers broad-spectrum resistance against soilborne sugar beet-infecting viruses from different families by recognizing triple gene block protein 1
Molecular Plant Pathology ( IF 4.8 ) Pub Date : 2021-05-05 , DOI: 10.1111/mpp.13066
Veronika Wetzel ₁ , Glenda Willlems ₂ , Aude Darracq ₂ , Yann Galein ₂ , Sebastian Liebe ₁ , Mark Varrelmann ₁

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Sugar beet cultivation is dependent on an effective control of beet necrotic yellow vein virus (BNYVV, family Benyviridae), which causes tremendous economic losses in sugar production. As the virus is transmitted by a soilborne protist, the use of resistant cultivars is currently the only way to control the disease. The Rz2 gene product belongs to a family of proteins conferring resistance towards diverse pathogens in plants. These proteins contain coiled-coil and leucine-rich repeat domains. After artificial inoculation of homozygous Rz2 resistant sugar beet lines, BNYVV and beet soilborne mosaic virus (BSBMV, family Benyviridae) were not detected. Analysis of the expression of Rz2 in naturally infected plants indicated constitutive expression in the root system. In a transient assay, coexpression of Rz2 and the individual BNYVV-encoded proteins revealed that only the combination of Rz2 and triple gene block protein 1 (TGB1) resulted in a hypersensitive reaction (HR)-like response. Furthermore, HR was also triggered by the TGB1 homologues from BSBMV as well as from the more distantly related beet soilborne virus (family Virgaviridae). This is the first report of an R gene providing resistance across different plant virus families.

中文翻译：

甜菜衍生的抗性基因 Rz2 通过识别三基因块蛋白 1，对不同科的土传甜菜感染病毒具有广谱抗性

甜菜种植依赖于对甜菜坏死黄脉病毒（BNYVV，本尼病毒科）的有效控制，该病毒给制糖业造成了巨大的经济损失。由于该病毒是通过土传原生生物传播的，因此使用抗性品种是目前控制该疾病的唯一方法。 Rz2基因产物属于赋予植物针对多种病原体的抗性的蛋白质家族。这些蛋白质含有卷曲螺旋和富含亮氨酸的重复结构域。人工接种Rz2抗性甜菜纯合品系后，未检出BNYVV和甜菜土传花叶病毒（BSBMV， Benyviridae科）。对自然感染植物中Rz2表达的分析表明在根系统中存在组成型表达。在瞬时测定中， Rz2和单个 BNYVV 编码蛋白的共表达表明，只有Rz2和三基因块蛋白 1 (TGB1) 的组合才会导致过敏反应 (HR) 样反应。此外，来自 BSBMV 以及关系较远的甜菜土传病毒（ Virgaviridae科）的 TGB1 同源物也可触发 HR。这是R基因对不同植物病毒家族提供抗性的第一份报告。

更新日期：2021-06-28

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