The cDNA of rbp1a, rbp2a, rbp2b, rbp5, and rbp7b genes was cloned and characterized in brown trout, and their mRNA expressions in response to different levels of dietary soybean oil (SBO) were determined along with their growth parameters, muscle fatty acid (FA) profile, and hepatic retinol level. Dietary SBO did not affect the growth parameters, survival, and hepatosomatic index. The FA composition and retinol content of fish reflected those of experimental diets. The retinol-binding proteins (Rbps; except Rbp5) contained lipocalin domains. The phylogenetic analysis showed that brown trout rbps are orthologs of the rbp/RBPs from other vertebrates; this was also supported by identity/similarity analysis. The rbp genes were exhibited to have conserved synteny with zebrafish and human rbps/RBPs. The tissue-specific transcription of duplicated rbp2a and rbp2b genes varied among all the tested tissues. Moreover, the diet including 100% SBO affected the rbp2a and rbp2b mRNA transcriptions differently, and this was explained by the divergence of the transcriptional regulation site of these genes due to the teleost-specific whole genome duplication. rbp1b and rbp7a were lost in the brown trout genome most likely because of nonfunctionalization. The mRNA expression of all the rbp genes studied was affected by dietary SBO. Therefore, their mRNA expressions could be modulated by dietary FAs and retinol content.

克隆并在棕鳟鱼中鉴定了rbp1a，rbp2a，rbp2b，rbp5和rbp7b基因的cDNA并对其进行了表征，并确定了其对不同水平日粮豆油（SBO）的响应的mRNA表达以及其生长参数肌肉脂肪酸（ FA）谱和肝视黄醇水平。饮食中的SBO不会影响生长参数，存活率和肝体指数。鱼的FA组成和视黄醇含量反映了实验饮食的含量。视黄醇结合蛋白（Rbps； Rbp5除外）包含脂质运载蛋白结构域。系统发育分析表明，褐鳟rbp是rbp / RBP的直系同源物。来自其他脊椎动物；同一性/相似性分析也支持了这一点。所述RBP基因表现出具有与斑马鱼和人保守的同线性限制性商业惯例/ RBP秒。在所有测试组织中，重复的rbp2a和rbp2b基因的组织特异性转录均不同。而且，含100％SBO的饮食对rbp2a和rbp2b mRNA转录的影响不同，这可以解释为由于硬骨特异性全基因组重复，这些基因的转录调控位点不同。rbp1b和rbp7a在褐鳟基因组中丢失的原因很可能是由于非功能化。饮食中的SBO影响所有研究的rbp基因的mRNA表达。因此，它们的mRNA表达可以通过饮食中的FA和视黄醇含量来调节。