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The tumour suppressor brain tumour (Brat) regulates linker histone dBigH1 expression in the Drosophila female germline and the early embryo
Open Biology ( IF 4.5 ) Pub Date : 2021-05-05 , DOI: 10.1098/rsob.200408
Paula Climent-Cantó 1, 2 , Albert Carbonell 1, 2 , Srividya Tamirisa 1, 2 , Laszlo Henn 3 , Salvador Pérez-Montero 1, 2 , Imre M Boros 3, 4 , Fernando Azorín 1, 2
Affiliation  

Linker histones H1 are essential chromatin components that exist as multiple developmentally regulated variants. In metazoans, specific H1s are expressed during germline development in a tightly regulated manner. However, the mechanisms governing their stage-dependent expression are poorly understood. Here, we address this question in Drosophila, which encodes for a single germline-specific dBigH1 linker histone. We show that during female germline lineage differentiation, dBigH1 is expressed in germ stem cells and cystoblasts, becomes silenced during transit-amplifying (TA) cystocytes divisions to resume expression after proliferation stops and differentiation starts, when it progressively accumulates in the oocyte. We find that dBigH1 silencing during TA divisions is post-transcriptional and depends on the tumour suppressor Brain tumour (Brat), an essential RNA-binding protein that regulates mRNA translation and stability. Like other oocyte-specific variants, dBigH1 is maternally expressed during early embryogenesis until it is replaced by somatic dH1 at the maternal-to-zygotic transition (MZT). Brat also mediates dBigH1 silencing at MZT. Finally, we discuss the situation in testes, where Brat is not expressed, but dBigH1 is translationally silenced too.



中文翻译:

肿瘤抑制脑肿瘤 (Brat) 调节连接组蛋白 dBigH1 在果蝇雌性生殖系和早期胚胎中的表达

接头组蛋白 H1 是重要的染色质成分,以多种发育调控的变体形式存在。在后生动物中,特定的 H1s 在种系发育过程中以一种严格调控的方式表达。然而,人们对控制其阶段依赖性表达的机制知之甚少。在这里,我们在果蝇中解决这个问题,它编码单个种系特异性 dBigH1 接头组蛋白。我们表明,在雌性生殖系谱系分化过程中,dBigH1 在生殖干细胞和成囊细胞中表达,在转运扩增 (TA) 囊细胞分裂过程中变得沉默,以在增殖停止和分化开始后恢复表达,此时它逐渐在卵母细胞中积累。我们发现 TA 分裂期间的 dBigH1 沉默是转录后的,并且取决于肿瘤抑制因子脑肿瘤 (Brat),这是一种调节 mRNA 翻译和稳定性的必需 RNA 结合蛋白。与其他卵母细胞特异性变体一样,dBigH1 在早期胚胎发生期间母体表达,直到在母体-合子转换 (MZT) 时被体细胞 dH1 取代。Brat 还介导 MZT 的 dBigH1 沉默。最后,我们讨论睾丸的情况,

更新日期:2021-05-05
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