Introduction: Alternative methods of dry storage and transportation may be a viable alternative to the use of liquid storage medium for cervical samples, especially for screening programs in places with few resources. Objective: The objective of this study is to verify the viability and efficacy of human papillomavirus DNA (HPV-DNA) detection in cervical cell samples collected and stored on a Flinders Technology Associates (FTA) card (Whatman Indicating FTA^® Elute Micro Card) and subsequently recovered in ethanol-based liquid medium and to compare the results to those obtained using samples stored directly in ethanol-based liquid medium. Study Design: Thirty-four women submitted to ETZ (excision of the transformation zone of the cervix) were included in this study. Before ETZ, 2 samples of exfoliated cervical cells were collected from each woman by a doctor and stored in ethanol-based liquid medium and on an FTA card. DNA recovery from FTA samples was performed using ethanol-based liquid medium. Detection of HPV-DNA in the samples was performed using the Cobas® 4800 HPV Test Platform. Results and Conclusions: The HPV-DNA detection positivity rates were 70.6% for the samples collected directly in liquid medium and 64.7% for the samples stored on the FTA card, with high detection accuracy in the DNA samples recovered from the FTA card (area under the curve = 0.958; 95% confidence interval = 0.890–1.000). The concordance between the results obtained using the 2 storage media was 94.1% (Kappa = 0.866). These preliminary results suggest that collection of cervical material on an FTA card may be an alternative to storage in liquid medium since the liquid medium has some limitations. In addition, DNA recovery from the card using ethanol-based liquid medium streamlines the workflow in the laboratory and reduces the cost associated with reagents, thereby facilitating access to the HPV test in places with few resources and potentially improving cervical cancer screening.
Acta Cytologica

中文翻译：

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使用FTA卡存储的样品中基于乙醇的液体培养基进行DNA回收以进行HPV检测

简介：干存储和运输的替代方法可能是将液体存储介质用于宫颈样本的一种可行的替代方法，尤其是在资源匮乏的地方进行筛查程序。目的：这项研究的目的是验证生存和人乳头瘤病毒DNA（HPV-DNA）检测收集并存储在弗林德斯技术协会（FTA），宫颈细胞样品中的功效卡（指示型FTA的Whatman ^®洗脱微卡）和随后在基于乙醇的液体介质中进行回收，并将结果与​​使用直接存储在基于乙醇的液体介质中的样品所获得的结果进行比较。学习规划：这项研究包括了34名接受ETZ（宫颈转换区切除术）的妇女。在进行ETZ之前，医生从每个女性那里收集了2个脱落的宫颈细胞样本，并存储在乙醇基液体培养基中和FTA卡上。使用乙醇基液体培养基从FTA样品中回收DNA。使用Cobas®4800 HPV测试平台对样品中的HPV-DNA进行检测。结果与结论：直接在液体培养基中收集的样品的HPV-DNA检测阳性率为70.6％，而在FTA卡上存储的样品的HPV-DNA检测阳性率为64.7％，从FTA卡回收的DNA样品的检测准确度很高（曲线下的面积= 0.958） ； 95％置信区间= 0.890–1.000）。使用两种存储介质获得的结果之间的一致性为94.1％（Kappa = 0.866）。这些初步结果表明，由于液体介质有一些局限性，因此在FTA卡上收集宫颈材料可能是液体介质中存储的一种替代选择。此外，使用基于乙醇的液体介质从卡中回收DNA可以简化实验室的工作流程，并降低与试剂相关的成本，
细胞学学报