Applicability of optoacoustic imaging in biology and medicine is determined by several key performance characteristics. In particular, an inherent trade-off exists between the acquired field-of-view (FOV) and temporal resolution of the measurements, which may hinder studies looking at rapid biodynamics at the whole-body level. Here, we report on a single-sweep volumetric optoacoustic tomography (sSVOT) system that attains whole body three-dimensional mouse scans within 1.8 s with better than 200 μm spatial resolution. sSVOT employs a spherical matrix array transducer in combination with multibeam illumination, the latter playing a critical role in maximizing the effective FOV and imaging speed performance. The system further takes advantage of the spatial response of the individual ultrasound detection elements to mitigate common image artifacts related to limited-view tomographic geometry, thus enabling rapid acquisitions without compromising image quality and contrast. We compare performance metrics to the previously reported whole-body mouse imaging implementations and alternative image compounding and reconstruction strategies. It is anticipated that sSVOT will open new venues for studying large-scale biodynamics, such as accumulation and clearance of molecular agents and drugs across multiple organs, circulation of cells, and functional responses to stimuli.

中文翻译：

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整只小鼠的单次扫描体积光声断层扫描

光声成像在生物学和医学中的适用性取决于几个关键的性能特征。特别是，在获得的视野 (FOV) 和测量的时间分辨率之间存在固有的权衡，这可能会阻碍在全身水平上研究快速生物动力学。在这里，我们报告了单次扫描体积光声断层扫描 (sSVOT) 系统，该系统可在 1.8 秒内以优于 200 μm 的空间分辨率实现全身三维鼠标扫描。sSVOT 采用球形矩阵阵列换能器与多光束照明相结合，后者在最大化有效 FOV 和成像速度性能方面发挥着关键作用。该系统进一步利用各个超声检测元件的空间响应来减轻与有限视野断层摄影几何相关的常见图像伪影，从而在不影响图像质量和对比度的情况下实现快速采集。我们将性能指标与之前报道的全身鼠标成像实现以及替代图像复合和重建策略进行了比较。预计 sSVOT 将为研究大规模生物动力学开辟新的场所，例如分子试剂和药物在多个器官中的积累和清除、细胞循环以及对刺激的功能反应。我们将性能指标与之前报道的全身鼠标成像实现以及替代图像复合和重建策略进行了比较。预计 sSVOT 将为研究大规模生物动力学开辟新的场所，例如分子试剂和药物在多个器官中的积累和清除、细胞循环以及对刺激的功能反应。我们将性能指标与之前报道的全身鼠标成像实现以及替代图像复合和重建策略进行了比较。预计 sSVOT 将为研究大规模生物动力学开辟新的场所，例如分子试剂和药物在多个器官中的积累和清除、细胞循环以及对刺激的功能反应。