ABSTRACT

The aim of the study was to verify the effect of bioaugmentation by the bacterial consortium YS with hydroxypropyl-β-cyclodextrin (HPCD) in a soil slurry. The bacterial consortium YS was enriched from a petroleum-polluted soil using pyrene as sole carbon resource. After 3 weeks, the degradation rate of phenanthrene in CK increased from 22.58% to 55.23 and 78.21% in bioaugmentation (B) and HPCD + bioaugmentation (MB) respectively. The degradation rate of pyrene in CK increased from 17.33% to 51.10% and 60.32% in B and MB respectively in the slurry. The augmented YS persisted in the slurry as monitored by 16S rRNA gene high-throughput sequencing and outcompeted some indigenous bacteria. Enhanced polycyclic aromatic hydrocarbon (PAH) degradation was observed in the addition of HPCD due to the enhanced bioavailability of phenanthrene and pyrene. Additionally, the amount of PAH-degrading bacteria and enzymatic activity in bioaugmentation with HPCD were higher than that in the CK group. The results indicated that bioaugmentation with a bacterial consortium and HPCD is an environmentally friendly method for the bioremediation of PAH-polluted soil.

摘要

该研究的目的是验证细菌聚生体 YS 与土壤浆液中的羟丙基-β-环糊精 (HPCD) 的生物强化效果。使用芘作为唯一的碳资源，从石油污染的土壤中富集了细菌聚生体 YS。3周后，在生物强化（B）和HPCD+生物强化（MB）中，CK中菲的降解率分别从22.58%增加到55.23和78.21%。浆料中 CK 中芘的降解率从 17.33% 提高到 B 和 MB 中的 51.10% 和 60.32%。通过 16S rRNA 基因高通量测序监测，增强的 YS 持续存在于浆液中，并胜过一些本土细菌。由于菲和芘的生物利用度增强，在添加 HPCD 时观察到增强的多环芳烃 (PAH) 降解。此外，HPCD 生物强化中 PAH 降解菌的数量和酶活性高于 CK 组。结果表明，细菌联合体和HPCD生物强化是一种环境友好的PAH污染土壤生物修复方法。