Abstract

Sanguinarine, a plant-derived benzophenanthridine alkaloid, was studied in terms of its anti-biofilm effects against carbapenem-resistant Serratia marcescens (CRSM). Minimum inhibitory concentrations (MICs) and cell membrane integrity were measured to investigate the antimicrobial mechanism of sanguinarine. Additionally, the extent of biofilm formation by CRSM exposed to sanguinarine was measured by crystal violet staining and visualized via field emission scanning electron microscopy and confocal laser scanning microscopy. Sanguinarine displayed moderate activity against CRSM, with a MIC₉₀ of 32 μg ml⁻¹. Moreover, cell membrane integrity was severely disrupted by sanguinarine at 64 μg ml⁻¹, and biofilm formation was sharply inhibited at 32 μg ml⁻¹. The minimum biofilm eradication concentration was 512 μg ml⁻¹ against mature CRSM biofilms. The overall results suggest that sanguinarine is a potential anti-biofilm agent that can be explored to treat CRSM infections.

摘要

从植物中的苯并菲啶生物碱Sanguinarine的抗生物膜作用出发，它对耐碳青霉烯的粘质沙雷氏菌（Serratia marcescens，CRSM）具有抗生物膜作用。测量最低抑菌浓度（MIC）和细胞膜完整性，以研究血红素的抗菌机制。另外，通过结晶紫染色测量CRSM暴露于血红素碱的生物膜形成的程度，并通过场发射扫描电子显微镜和共聚焦激光扫描显微镜观察。血红素碱显示出对CRSM的中等活性，MIC ₉₀为32μgml ^-1。而且，在64μgml ^-1下，血红素碱严重破坏了细胞膜的完整性。，并且在32μgml ^-1时，生物膜的形成受到了明显的抑制。相对于成熟的CRSM生物膜，最小生物膜根除浓度为512μgml ^-1。总体结果表明，血红素碱是一种潜在的抗生物膜药物，可用于治疗CRSM感染。