Abstract

Salmonella biofilm prevention and control is of great importance. This study, investigated the use of the isolated phage KM16 belonging to the family Myoviridae in the order Caudovirales. The phage genome size was 170,126 bp. Almost all phages were adsorbed to the host within 20 min. KM16 had a latent period of 70 min followed by a rise period of 40 min. Phage KM16 had the ability to lytically infect 10 out of the 12 clinical strains of S. paratyphi tested. Phylogenetic analysis indicated that the S. paratyphi 16S rRNA, crispr 1 and fimA genes correlated with the lytic spectrum of phage KM16. The lytic spectrum of phage KM16 correlated with Salmonella pili (fimA), and Salmonella pili were the recognition site for phage adsorption to the host. Phage KM16 (MOI = 0.1) had a better anti-biofilm effect than kanamycin sulfate (10 ug ml⁻¹) in high-concentration Salmonella cultures.

摘要

沙门氏菌生物膜的预防和控制非常重要。这项研究以Caudovirales的顺序调查了属于Myoviridae家族的分离噬菌体KM16的使用。噬菌体基因组大小为170,126  bp。在20 分钟内，几乎所有噬菌体均吸附到宿主上。KM16的潜伏期为70 分钟，随后的上升期为40 分钟。噬菌体KM16能够裂解感染的12株副伤寒链球菌临床菌株中的10株。系统发育分析表明，副伤寒沙门氏菌16S rRNA ，crispr 1和fimA基因与噬菌体KM16的裂解谱相关。噬菌体KM16与沙门氏菌相关的裂解光谱pili（fimA）和沙门氏菌pili是噬菌体吸附到宿主的识别位点。在高浓度沙门氏菌培养物中，噬菌体KM16（MOI  =  0.1）具有比硫酸卡那霉素（10 ug ml ^-1）更好的抗生物膜作用。