♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus, a hybrid grouper created from artificial breeding, has been widely developed over the past decades. However, the study focusing on lukewarm high-protein-content fish species using advanced techniques has rarely been reported. In this work, the TMT (tandem mass tag)-assisted technique was employed to explore its differentially expressed proteins and response mechanisms under low-temperature dormant and waterless stresses. Our findings suggest that 162 and 258 differentially expressed proteins were identified under low-temperature dormant and waterless stresses, respectively. The waterless preservation treatment further identifies 93 differentially expressed proteins. The identified proteins are categorized and found to participate in lipid metabolism, glycometabolism, oxidative stress, immune response, protein and amino acid metabolism, signal transduction, and other functions. Accordingly, the factors that affect the response mechanisms are highlighted to provide new evidences at protein level.

♀ Epinephelus fuscoguttatu s × ♂ Epinephelus lanceolatus由人工繁育而成的杂交石斑鱼，在过去的几十年里得到了广泛的发展。然而，很少报道使用先进技术对温热的高蛋白含量鱼类进行的研究。在这项工作中，采用TMT（串联质量标签）辅助技术探索其在低温休眠和无水胁迫下的差异表达蛋白质和响应机制。我们的研究结果表明，分别在低温休眠和无水胁迫下鉴定出 162 和 258 个差异表达的蛋白质。无水保存处理进一步鉴定了 93 种差异表达的蛋白质。对鉴定出的蛋白质进行分类并发现参与脂质代谢、糖代谢、氧化应激、免疫反应、蛋白质和氨基酸代谢、信号转导等功能。因此，突出影响反应机制的因素，以提供蛋白质水平的新证据。